ID NS8B_SARS Reviewed; 84 AA.
AC Q80H93; Q7TFA6;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 02-OCT-2024, entry version 74.
DE RecName: Full=ORF8b protein;
DE AltName: Full=Accessory protein 8b;
DE AltName: Full=Non-structural protein 8b;
DE Short=ns8b;
GN ORFNames=8b;
OS Severe acute respiratory syndrome coronavirus (SARS-CoV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Betacoronavirus; Sarbecovirus.
OX NCBI_TaxID=694009;
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9675; Paguma larvata (Masked palm civet).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Urbani;
RX PubMed=12730500; DOI=10.1126/science.1085952;
RA Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R.,
RA Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S.,
RA Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D.,
RA Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S.,
RA Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R.,
RA Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J.,
RA Bellini W.J.;
RT "Characterization of a novel coronavirus associated with severe acute
RT respiratory syndrome.";
RL Science 300:1394-1399(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Tor2;
RX PubMed=12730501; DOI=10.1126/science.1085953;
RA Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A.,
RA Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y.,
RA Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R.,
RA Mayo M., McDonald H., Montgomery S.B., Pandoh P.K., Petrescu A.S.,
RA Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M.,
RA Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K.,
RA Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R.,
RA Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A.,
RA Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S.,
RA Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M.,
RA Skowronski D.M., Upton C., Roper R.L.;
RT "The genome sequence of the SARS-associated coronavirus.";
RL Science 300:1399-1404(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate CUHK-Su10, and Isolate CUHK-W1;
RX PubMed=12853594; DOI=10.1056/nejm200307103490216;
RA Tsui S.K.W., Chim S.S.C., Lo Y.M.D.;
RT "Coronavirus genomic-sequence variations and the epidemiology of the severe
RT acute respiratory syndrome.";
RL N. Engl. J. Med. 349:187-188(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate HKU-39849;
RX PubMed=12876307; DOI=10.1177/15353702-0322807-13;
RA Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C.,
RA Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B.,
RA Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.;
RT "The complete genome sequence of severe acute respiratory syndrome
RT coronavirus strain HKU-39849 (HK-39).";
RL Exp. Biol. Med. 228:866-873(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04, and
RC Isolate GD01;
RA Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T.,
RA Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F.,
RA Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W.,
RA Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W.,
RA Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L.,
RA Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J.,
RA Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H.;
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate TW1;
RA Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.;
RT "The complete genome of SARS coronavirus clone TW1.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate FRA;
RA Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S.,
RA Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J.,
RA Song H.C., Abrignani S., Covacci A., Rappuoli R.;
RT "SARS virus is a close relative of type II coronaviruses.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Frankfurt 1;
RA Thiel V., Hertzig T., Putics A., Ivanov K.A., Schelle B., Bayer S.,
RA Scheiner B., Weinand H., Weissbrich B., Ziebuhr J.;
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Taiwan TC1, Isolate Taiwan TC2, and Isolate Taiwan TC3;
RA Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.;
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [10]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS, and Isolate TWY;
RA Shu H.Y., Wu K.M., Tsai S.F.;
RT "The complete genome of SARS coronavirus TWH.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [11]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate HSR 1;
RA Canducci F., Clementi M., Poli G., Vicenzi E.;
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [12]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate AS;
RA Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M.,
RA Ruan Y.J., Salemi M.;
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [13]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Shanghai LY;
RA Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.;
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [14]
RP SUBCELLULAR LOCATION.
RC STRAIN=Isolate CUHK-Su10;
RX PubMed=16753150; DOI=10.1016/j.febslet.2006.05.051;
RA Law P.Y.P., Liu Y.-M., Geng H., Kwan K.H., Waye M.M.-Y., Ho Y.-Y.;
RT "Expression and functional characterization of the putative protein 8b of
RT the severe acute respiratory syndrome-associated coronavirus.";
RL FEBS Lett. 580:3643-3648(2006).
CC -!- FUNCTION: Non-structural protein which is dispensable for virus
CC replication in cell culture.
CC -!- INTERACTION:
CC Q80H93; Q96P20: NLRP3; Xeno; NbExp=7; IntAct=EBI-25492924, EBI-6253230;
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:16753150}.
CC Host nucleus {ECO:0000269|PubMed:16753150}.
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DR EMBL; AY278741; AAP13450.1; -; Genomic_RNA.
DR EMBL; AY274119; AAP41046.1; -; Genomic_RNA.
DR EMBL; AY282752; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY278554; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY278491; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY278487; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY278488; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY278489; AAP51236.1; ALT_SEQ; Genomic_RNA.
DR EMBL; AY278490; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY279354; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY310120; AAP50494.1; -; Genomic_RNA.
DR EMBL; AY291315; AAP33706.1; -; Genomic_RNA.
DR EMBL; AY348314; AAP97889.1; -; Genomic_RNA.
DR EMBL; AY338174; AAQ01604.1; -; Genomic_RNA.
DR EMBL; AY338175; AAQ01616.1; -; Genomic_RNA.
DR EMBL; AP006557; BAC81357.1; -; Genomic_RNA.
DR EMBL; AP006558; BAC81371.1; -; Genomic_RNA.
DR EMBL; AP006559; BAC81385.1; -; Genomic_RNA.
DR EMBL; AP006560; BAC81399.1; -; Genomic_RNA.
DR EMBL; AP006561; BAC81413.1; -; Genomic_RNA.
DR EMBL; AY323977; AAP72983.1; -; Genomic_RNA.
DR EMBL; AY427439; AAQ94069.1; -; Genomic_RNA.
DR EMBL; AH012999; AAP82973.1; -; Genomic_RNA.
DR BioGRID; 4383923; 60.
DR IntAct; Q80H93; 51.
DR MINT; Q80H93; -.
DR Reactome; R-HSA-9679504; Translation of Replicase and Assembly of the Replication Transcription Complex.
DR Reactome; R-HSA-9692916; SARS-CoV-1 activates/modulates innate immune responses.
DR SIGNOR; Q80H93; -.
DR Proteomes; UP000000354; Segment.
DR Proteomes; UP000103670; Segment.
DR Proteomes; UP000109640; Segment.
DR Proteomes; UP000116947; Segment.
DR Proteomes; UP000121636; Segment.
DR Proteomes; UP000131569; Segment.
DR Proteomes; UP000131955; Segment.
DR Proteomes; UP000137377; Genome.
DR Proteomes; UP000138690; Segment.
DR Proteomes; UP000143093; Segment.
DR Proteomes; UP000146108; Segment.
DR Proteomes; UP000146181; Segment.
DR Proteomes; UP000146296; Segment.
DR Proteomes; UP000148194; Segment.
DR Proteomes; UP000153467; Segment.
DR Proteomes; UP000160648; Segment.
DR Proteomes; UP000172416; Segment.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR InterPro; IPR022722; ORF8_betacoronavirus.
DR InterPro; IPR046444; SARS_ORF8_IG.
DR Pfam; PF12093; bCoV_NS8; 1.
DR PROSITE; PS51964; SARS_ORF8_IG; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Host cytoplasm; Host nucleus; Reference proteome.
FT CHAIN 1..84
FT /note="ORF8b protein"
FT /id="PRO_0000106135"
FT DOMAIN 1..82
FT /note="SARS ORF8 Ig-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01309"
FT DISULFID 22..40
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01309"
FT VARIANT 76
FT /note="L -> F (in strain: Isolate GD01)"
SQ SEQUENCE 84 AA; 9560 MW; 510EEE64470C3B1E CRC64;
MCLKILVRYN TRGNTYSTAW LCALGKVLPF HRWHTMVQTC TPNVTINCQD PAGGALIARC
WYLHEGHQTA AFRDVLVVLN KRTN
//