ID VEMP_SARS Reviewed; 76 AA.
AC P59637; Q7TA13; Q7TFA8;
DT 23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 23-APR-2003, sequence version 1.
DT 02-OCT-2024, entry version 115.
DE RecName: Full=Envelope small membrane protein {ECO:0000255|HAMAP-Rule:MF_04204};
DE Short=E protein {ECO:0000255|HAMAP-Rule:MF_04204};
DE Short=sM protein {ECO:0000255|HAMAP-Rule:MF_04204};
GN Name=E {ECO:0000255|HAMAP-Rule:MF_04204}; Synonyms=sM; ORFNames=4;
OS Severe acute respiratory syndrome coronavirus (SARS-CoV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Betacoronavirus; Sarbecovirus.
OX NCBI_TaxID=694009;
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9675; Paguma larvata (Masked palm civet).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Urbani;
RX PubMed=12730500; DOI=10.1126/science.1085952;
RA Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R.,
RA Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S.,
RA Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D.,
RA Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S.,
RA Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R.,
RA Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J.,
RA Bellini W.J.;
RT "Characterization of a novel coronavirus associated with severe acute
RT respiratory syndrome.";
RL Science 300:1394-1399(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Tor2;
RX PubMed=12730501; DOI=10.1126/science.1085953;
RA Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A.,
RA Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y.,
RA Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R.,
RA Mayo M., McDonald H., Montgomery S.B., Pandoh P.K., Petrescu A.S.,
RA Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M.,
RA Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K.,
RA Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R.,
RA Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A.,
RA Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S.,
RA Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M.,
RA Skowronski D.M., Upton C., Roper R.L.;
RT "The genome sequence of the SARS-associated coronavirus.";
RL Science 300:1399-1404(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate CUHK-Su10, and Isolate CUHK-W1;
RX PubMed=12853594; DOI=10.1056/nejm200307103490216;
RA Tsui S.K.W., Chim S.S.C., Lo Y.M.D.;
RT "Coronavirus genomic-sequence variations and the epidemiology of the severe
RT acute respiratory syndrome.";
RL N. Engl. J. Med. 349:187-188(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate GZ50, and Isolate HKU-36871;
RX PubMed=12958366; DOI=10.1126/science.1087139;
RA Guan Y., Zheng B.J., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S.W.,
RA Li P.H., Zhang L.J., Guan Y.J., Butt K.M., Wong K.L., Chan K.W., Lim W.,
RA Shortridge K.F., Yuen K.Y., Peiris J.S.M., Poon L.L.M.;
RT "Isolation and characterization of viruses related to the SARS coronavirus
RT from animals in southern China.";
RL Science 302:276-278(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate HKU-39849;
RX PubMed=12876307; DOI=10.1177/15353702-0322807-13;
RA Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C.,
RA Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B.,
RA Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.;
RT "The complete genome sequence of severe acute respiratory syndrome
RT coronavirus strain HKU-39849 (HK-39).";
RL Exp. Biol. Med. 228:866-873(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Sin2500, Isolate Sin2677, Isolate Sin2679, Isolate Sin2748,
RC and Isolate sin2774;
RX PubMed=12781537; DOI=10.1016/s0140-6736(03)13414-9;
RA Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y.,
RA Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L.,
RA Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T.;
RT "Comparative full-length genome sequence analysis of 14 SARS coronavirus
RT isolates and common mutations associated with putative origins of
RT infection.";
RL Lancet 361:1779-1785(2003).
RN [7]
RP ERRATUM OF PUBMED:12781537.
RA Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y.,
RA Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L.,
RA Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T.;
RL Lancet 361:1832-1832(2003).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04, and
RC Isolate GD01;
RA Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T.,
RA Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F.,
RA Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W.,
RA Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W.,
RA Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L.,
RA Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J.,
RA Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H.;
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate TW1;
RA Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.;
RT "The complete genome of SARS coronavirus clone TW1.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [10]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate FRA;
RA Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S.,
RA Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J.,
RA Song H.C., Abrignani S., Covacci A., Rappuoli R.;
RT "SARS virus is a close relative of type II coronaviruses.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [11]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Frankfurt 1;
RX PubMed=12917450; DOI=10.1099/vir.0.19424-0;
RA Thiel V., Ivanov K.A., Putics A., Hertzig T., Schelle B., Bayer S.,
RA Weissbrich B., Snijder E.J., Rabenau H., Doerr H.W., Gorbalenya A.E.,
RA Ziebuhr J.;
RT "Mechanisms and enzymes involved in SARS coronavirus genome expression.";
RL J. Gen. Virol. 84:2305-2315(2003).
RN [12]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate ZJ01;
RX PubMed=14527350;
RA Li L., Wang Z., Lu Y., Bao Q., Chen S., Wu N., Cheng S., Weng J., Zhang Y.,
RA Yan J., Mei L., Wang X., Zhu H., Yu Y., Zhang M., Li M., Yao J., Lu Q.,
RA Yao P., Bo X., Wo J., Wang S., Hu S.;
RT "Severe acute respiratory syndrome-associated coronavirus genotype and its
RT characterization.";
RL Chin. Med. J. 116:1288-1292(2003).
RN [13]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Isolate ZJ01;
RA Cong L.-M., Ding G.-Q., Lu Y.-Y., Weng J.-Q., Yan J.-Y., Hu N.-P.,
RA Wo J.-E., Chen S.-Y., Zhang Y.-J., Mei L.-L., Wang Z.-G., Yao J.,
RA Zhu H.-P., Lu Q.-Y., Li M.-H., Gong L.-M., Shi W., Li L.-J.;
RT "SARS coronavirus ZJ01 isolate small envelope E protein.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [14]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate TWC;
RA Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee S.C., Lin Y.-C.,
RA Hsu C.-K., Chen H.-Y., Chang J.G., Chen P.-J., Su I.-J.;
RT "Genomic sequence of SARS isolate from the first fatal case in Taiwan.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [15]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Shanghai QXC1;
RA Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.;
RT "Analysis of SARS coronavirus genome in Shanghai isolates.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [16]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Taiwan TC1, Isolate Taiwan TC2, and Isolate Taiwan TC3;
RA Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.;
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [17]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS, and Isolate TWY;
RA Shu H.Y., Wu K.M., Tsai S.F.;
RT "The complete genome of SARS coronavirus TWH.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [18]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate HSR 1;
RA Canducci F., Clementi M., Poli G., Vicenzi E.;
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [19]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate TWC2, and Isolate TWC3;
RA Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee H.-C., Lin Y.-C.,
RA Hsu C.-K., Chen H.-Y., Chen P.-J., Su I.-J.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [20]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate AS;
RA Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M.,
RA Ruan Y.J., Salemi M.;
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [21]
RP INTERACTION WITH ACCESSORY PROTEIN 3A.
RX PubMed=15194747; DOI=10.1128/jvi.78.13.6723-6734.2004;
RA Tan Y.-J., Teng E., Shen S., Tan T.H.P., Goh P.-Y., Fielding B.C.,
RA Ooi E.-E., Tan H.-C., Lim S.G., Hong W.;
RT "A novel severe acute respiratory syndrome coronavirus protein, U274, is
RT transported to the cell surface and undergoes endocytosis.";
RL J. Virol. 78:6723-6734(2004).
RN [22]
RP INTERACTION WITH ACCESSORY PROTEIN 3A.
RX PubMed=15763150; DOI=10.1016/j.virusres.2005.01.001;
RA Yuan X., Li J., Shan Y., Yang Z., Zhao Z., Chen B., Yao Z., Dong B.,
RA Wang S., Chen J., Cong Y.;
RT "Subcellular localization and membrane association of SARS-CoV 3a
RT protein.";
RL Virus Res. 109:191-202(2005).
RN [23]
RP INTERACTION WITH ACCESSORY PROTEIN 7A.
RX PubMed=16580632; DOI=10.1016/j.bbrc.2006.03.091;
RA Fielding B.C., Gunalan V., Tan T.H.P., Chou C.-F., Shen S., Khan S.,
RA Lim S.G., Hong W., Tan Y.-J.;
RT "Severe acute respiratory syndrome coronavirus protein 7a interacts with
RT hSGT.";
RL Biochem. Biophys. Res. Commun. 343:1201-1208(2006).
RN [24]
RP INTERACTION WITH PROTEIN M.
RX PubMed=19322648; DOI=10.1007/s11262-009-0341-6;
RA Chen S.C., Lo S.Y., Ma H.C., Li H.C.;
RT "Expression and membrane integration of SARS-CoV E protein and its
RT interaction with M protein.";
RL Virus Genes 38:365-371(2009).
RN [25]
RP INTERACTION WITH HUMAN PALS1, SUBCELLULAR LOCATION, MOTIF, AND MUTAGENESIS
RP OF 73-ASP--VAL-76.
RX PubMed=20861307; DOI=10.1091/mbc.e10-04-0338;
RA Teoh K.T., Siu Y.L., Chan W.L., Schlueter M.A., Liu C.J., Peiris J.S.,
RA Bruzzone R., Margolis B., Nal B.;
RT "The SARS coronavirus E protein interacts with PALS1 and alters tight
RT junction formation and epithelial morphogenesis.";
RL Mol. Biol. Cell 21:3838-3852(2010).
RN [26]
RP SUBCELLULAR LOCATION.
RX PubMed=21450821; DOI=10.1128/jvi.00060-11;
RA Cohen J.R., Lin L.D., Machamer C.E.;
RT "Identification of a Golgi complex-targeting signal in the cytoplasmic tail
RT of the severe acute respiratory syndrome coronavirus envelope protein.";
RL J. Virol. 85:5794-5803(2011).
RN [27]
RP SUBUNIT.
RX PubMed=22819936; DOI=10.1016/j.pep.2012.07.005;
RA Parthasarathy K., Lu H., Surya W., Vararattanavech A., Pervushin K.,
RA Torres J.;
RT "Expression and purification of coronavirus envelope proteins using a
RT modified beta-barrel construct.";
RL Protein Expr. Purif. 85:133-141(2012).
RN [28]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=24788150; DOI=10.1371/journal.ppat.1004077;
RA Nieto-Torres J.L., DeDiego M.L., Verdia-Baguena C., Jimenez-Guardeno J.M.,
RA Regla-Nava J.A., Fernandez-Delgado R., Castano-Rodriguez C., Alcaraz A.,
RA Torres J., Aguilella V.M., Enjuanes L.;
RT "Severe acute respiratory syndrome coronavirus envelope protein ion channel
RT activity promotes virus fitness and pathogenesis.";
RL PLoS Pathog. 10:E1004077-E1004077(2014).
RN [29]
RP INTERACTION WITH NUCLEOPROTEIN.
RX PubMed=24766657; DOI=10.1186/1423-0127-21-34;
RA Tseng Y.T., Wang S.M., Huang K.J., Wang C.T.;
RT "SARS-CoV envelope protein palmitoylation or nucleocapsid association is
RT not required for promoting virus-like particle production.";
RL J. Biomed. Sci. 21:34-34(2014).
RN [30]
RP FUNCTION.
RX PubMed=26331680; DOI=10.1016/j.virol.2015.08.010;
RA Nieto-Torres J.L., Verdia-Baguena C., Jimenez-Guardeno J.M.,
RA Regla-Nava J.A., Castano-Rodriguez C., Fernandez-Delgado R., Torres J.,
RA Aguilella V.M., Enjuanes L.;
RT "Severe acute respiratory syndrome coronavirus E protein transports calcium
RT ions and activates the NLRP3 inflammasome.";
RL Virology 485:330-339(2015).
CC -!- FUNCTION: Plays a central role in virus morphogenesis and assembly.
CC Acts as a viroporin and self-assembles in host membranes forming
CC pentameric protein-lipid pores that allow ion transport. Also plays a
CC role in the induction of apoptosis (By similarity). Activates the host
CC NLRP3 inflammasome, leading to IL-1beta overproduction.
CC {ECO:0000255|HAMAP-Rule:MF_04204, ECO:0000269|PubMed:24788150,
CC ECO:0000269|PubMed:26331680}.
CC -!- SUBUNIT: Homopentamer (PubMed:22819936). Interacts with membrane
CC protein M in the budding compartment of the host cell, which is located
CC between endoplasmic reticulum and the Golgi complex (PubMed:19322648).
CC Interacts with Nucleoprotein (PubMed:24766657). Interacts with the
CC accessory proteins 3a and 7a. Interacts (via C-terminus) with human
CC PALS1 (via PDZ domain); this inhibits the interaction between human
CC PALS1 and human CRB3, and causes delayed tight junction formation and
CC defective cell polarity (PubMed:20861307). {ECO:0000255|HAMAP-
CC Rule:MF_04204, ECO:0000269|PubMed:15194747,
CC ECO:0000269|PubMed:15763150, ECO:0000269|PubMed:16580632,
CC ECO:0000269|PubMed:20861307, ECO:0000269|PubMed:22819936}.
CC -!- INTERACTION:
CC P59637; Q7TFA1: 7b; NbExp=2; IntAct=EBI-25487741, EBI-25492846;
CC P59637; P59637: E; NbExp=3; IntAct=EBI-25487741, EBI-25487741;
CC P59637; P59596: M; NbExp=6; IntAct=EBI-25487741, EBI-25487824;
CC P59637; P59595: N; NbExp=4; IntAct=EBI-25487741, EBI-7602718;
CC P59637; PRO_0000037309 [P0C6X7]: rep; NbExp=2; IntAct=EBI-25487741, EBI-25475797;
CC P59637; PRO_0000037311 [P0C6X7]: rep; NbExp=5; IntAct=EBI-25487741, EBI-25474079;
CC P59637; PRO_0000037315 [P0C6X7]: rep; NbExp=2; IntAct=EBI-25487741, EBI-25487941;
CC P59637; O35843: Bcl2l1; Xeno; NbExp=2; IntAct=EBI-25487741, EBI-25487771;
CC P59637; Q64373: Bcl2l1; Xeno; NbExp=2; IntAct=EBI-25487741, EBI-526361;
CC P59637; Q8N3R9: PALS1; Xeno; NbExp=4; IntAct=EBI-25487741, EBI-2513978;
CC P59637; Q8N3R9-1: PALS1; Xeno; NbExp=9; IntAct=EBI-25487741, EBI-8231026;
CC P59637; O00560-1: SDCBP; Xeno; NbExp=4; IntAct=EBI-25487741, EBI-9640690;
CC -!- SUBCELLULAR LOCATION: Host endoplasmic reticulum-Golgi intermediate
CC compartment {ECO:0000269|PubMed:20861307}. Host Golgi apparatus
CC membrane {ECO:0000255|HAMAP-Rule:MF_04204, ECO:0000269|PubMed:21450821,
CC ECO:0000269|PubMed:24788150}; Single-pass type III membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_04204}. Note=Colocalizes with S in the host
CC endoplasmic reticulum-Golgi intermediate compartment (PubMed:20861307).
CC The cytoplasmic tail functions as a Golgi complex-targeting signal.
CC {ECO:0000255|HAMAP-Rule:MF_04204, ECO:0000269|PubMed:20861307,
CC ECO:0000269|PubMed:21450821}.
CC -!- SIMILARITY: Belongs to the betacoronaviruses E protein family.
CC {ECO:0000255|HAMAP-Rule:MF_04204}.
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DR EMBL; AY278741; AAP13443.1; -; Genomic_RNA.
DR EMBL; AY274119; AAP41040.1; -; Genomic_RNA.
DR EMBL; AY304492; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY282752; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY278554; AAP13570.1; -; Genomic_RNA.
DR EMBL; AY278491; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY283794; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY283795; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY283796; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY283797; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY283798; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY278487; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY278488; AAP30033.1; -; Genomic_RNA.
DR EMBL; AY278489; AAP51230.1; -; Genomic_RNA.
DR EMBL; AY278490; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY279354; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY291451; AAP37020.1; -; Genomic_RNA.
DR EMBL; AY310120; AAP50488.1; -; Genomic_RNA.
DR EMBL; AY291315; AAP33700.1; -; Genomic_RNA.
DR EMBL; AY297028; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY323975; AAP73416.1; -; mRNA.
DR EMBL; AY321118; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY463059; AAP82979.2; -; Genomic_RNA.
DR EMBL; AY338174; AAQ01600.1; -; Genomic_RNA.
DR EMBL; AY338175; AAQ01612.1; -; Genomic_RNA.
DR EMBL; AY348314; AAP97885.1; -; Genomic_RNA.
DR EMBL; AP006557; BAC81351.1; -; Genomic_RNA.
DR EMBL; AP006558; BAC81365.1; -; Genomic_RNA.
DR EMBL; AP006559; BAC81379.1; -; Genomic_RNA.
DR EMBL; AP006560; BAC81393.1; -; Genomic_RNA.
DR EMBL; AP006561; BAC81407.1; -; Genomic_RNA.
DR EMBL; AY323977; AAP72977.1; -; Genomic_RNA.
DR EMBL; AY362698; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY362699; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY427439; AAQ94063.1; -; Genomic_RNA.
DR PDB; 2MM4; NMR; -; A=8-65.
DR PDB; 5X29; NMR; -; A/B/C/D/E=8-65.
DR PDB; 5XER; NMR; -; A=55-63.
DR PDB; 5XES; NMR; -; A=55-63.
DR PDB; 7NTJ; X-ray; 1.74 A; C/G=69-76.
DR PDBsum; 2MM4; -.
DR PDBsum; 5X29; -.
DR PDBsum; 5XER; -.
DR PDBsum; 5XES; -.
DR PDBsum; 7NTJ; -.
DR SMR; P59637; -.
DR BioGRID; 4383914; 43.
DR IntAct; P59637; 34.
DR OrthoDB; 38268at10239; -.
DR Reactome; R-HSA-9678110; Attachment and Entry.
DR Reactome; R-HSA-9679509; Virion Assembly and Release.
DR Reactome; R-HSA-9683683; Maturation of protein E.
DR Reactome; R-HSA-9683701; Translation of Structural Proteins.
DR Reactome; R-HSA-9692912; SARS-CoV-1 targets PDZ proteins in cell-cell junction.
DR Reactome; R-HSA-9692913; SARS-CoV-1-mediated effects on programmed cell death.
DR Reactome; R-HSA-9692916; SARS-CoV-1 activates/modulates innate immune responses.
DR SIGNOR; P59637; -.
DR EvolutionaryTrace; P59637; -.
DR Proteomes; UP000000354; Segment.
DR Proteomes; UP000103670; Segment.
DR Proteomes; UP000109640; Segment.
DR Proteomes; UP000116947; Segment.
DR Proteomes; UP000121636; Segment.
DR Proteomes; UP000131569; Segment.
DR Proteomes; UP000131955; Segment.
DR Proteomes; UP000137377; Genome.
DR Proteomes; UP000138690; Segment.
DR Proteomes; UP000143093; Segment.
DR Proteomes; UP000145651; Segment.
DR Proteomes; UP000146108; Segment.
DR Proteomes; UP000146181; Segment.
DR Proteomes; UP000146296; Segment.
DR Proteomes; UP000148194; Segment.
DR Proteomes; UP000153467; Segment.
DR Proteomes; UP000160648; Segment.
DR Proteomes; UP000164441; Segment.
DR Proteomes; UP000172416; Segment.
DR GO; GO:0044172; C:host cell endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
DR GO; GO:0044177; C:host cell Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0055036; C:virion membrane; TAS:Reactome.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0051673; P:disruption of plasma membrane integrity in another organism; IDA:CACAO.
DR GO; GO:0046760; P:viral budding from Golgi membrane; IDA:UniProtKB.
DR CDD; cd21536; SARS-CoV-2_E; 1.
DR Gene3D; 6.10.250.1810; -; 1.
DR HAMAP; MF_04204; BETA_CORONA_E; 1.
DR InterPro; IPR043506; E_protein_bCoV.
DR InterPro; IPR003873; E_protein_CoV.
DR InterPro; IPR044377; E_SARS-CoV-2.
DR Pfam; PF02723; CoV_E; 1.
DR PROSITE; PS51926; COV_E; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Apoptosis; Host Golgi apparatus; Host membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..76
FT /note="Envelope small membrane protein"
FT /id="PRO_0000106074"
FT TOPO_DOM 1..16
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04204"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04204"
FT TOPO_DOM 38..76
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04204"
FT MOTIF 73..76
FT /note="PDZ-binding; required for interaction with human
FT PALS1"
FT /evidence="ECO:0000269|PubMed:20861307"
FT VARIANT 24
FT /note="V -> M (in strain: Isolate GD01)"
FT MUTAGEN 73..76
FT /note="Missing: Abolishes interaction with PDZ domain of
FT human PALS1; loss of accumulation in host perinuclear
FT compartment."
FT /evidence="ECO:0000269|PubMed:20861307"
FT HELIX 14..42
FT /evidence="ECO:0007829|PDB:2MM4"
FT TURN 43..45
FT /evidence="ECO:0007829|PDB:2MM4"
FT HELIX 56..64
FT /evidence="ECO:0007829|PDB:2MM4"
SQ SEQUENCE 76 AA; 8361 MW; A6D9A31652DDA194 CRC64;
MYSFVSEETG TLIVNSVLLF LAFVVFLLVT LAILTALRLC AYCCNIVNVS LVKPTVYVYS
RVKNLNSSEG VPDLLV
//