ID NS3B_SARS Reviewed; 154 AA. AC P59633; Q7TFA9; Q80BV4; DT 23-APR-2003, integrated into UniProtKB/Swiss-Prot. DT 23-APR-2003, sequence version 1. DT 24-JUL-2024, entry version 88. DE RecName: Full=ORF3b protein; DE AltName: Full=Accessory protein 3b; DE AltName: Full=Non-structural protein 3b; DE Short=ns3b; DE AltName: Full=Protein X2; GN ORFNames=3b; OS Severe acute respiratory syndrome coronavirus (SARS-CoV). OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; OC Betacoronavirus; Sarbecovirus. OX NCBI_TaxID=694009; OH NCBI_TaxID=9606; Homo sapiens (Human). OH NCBI_TaxID=9675; Paguma larvata (Masked palm civet). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate Urbani; RX PubMed=12730500; DOI=10.1126/science.1085952; RA Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R., RA Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S., RA Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D., RA Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S., RA Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R., RA Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J., RA Bellini W.J.; RT "Characterization of a novel coronavirus associated with severe acute RT respiratory syndrome."; RL Science 300:1394-1399(2003). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate Tor2; RX PubMed=12730501; DOI=10.1126/science.1085953; RA Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A., RA Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y., RA Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R., RA Mayo M., McDonald H., Montgomery S.B., Pandoh P.K., Petrescu A.S., RA Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M., RA Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K., RA Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R., RA Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A., RA Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S., RA Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M., RA Skowronski D.M., Upton C., Roper R.L.; RT "The genome sequence of the SARS-associated coronavirus."; RL Science 300:1399-1404(2003). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate CUHK-Su10, and Isolate CUHK-W1; RX PubMed=12853594; DOI=10.1056/nejm200307103490216; RA Tsui S.K.W., Chim S.S.C., Lo Y.M.D.; RT "Coronavirus genomic-sequence variations and the epidemiology of the severe RT acute respiratory syndrome."; RL N. Engl. J. Med. 349:187-188(2003). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate HKU-39849; RX PubMed=12876307; DOI=10.1177/15353702-0322807-13; RA Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C., RA Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B., RA Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.; RT "The complete genome sequence of severe acute respiratory syndrome RT coronavirus strain HKU-39849 (HK-39)."; RL Exp. Biol. Med. 228:866-873(2003). RN [5] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04, and RC Isolate GD01; RA Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T., RA Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F., RA Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W., RA Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W., RA Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L., RA Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J., RA Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H.; RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate TW1; RA Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.; RT "The complete genome of SARS coronavirus clone TW1."; RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. RN [7] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate FRA; RA Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S., RA Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J., RA Song H.C., Abrignani S., Covacci A., Rappuoli R.; RT "SARS virus is a close relative of type II coronaviruses."; RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. RN [8] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate Frankfurt 1; RA Thiel V., Hertzig T., Putics A., Ivanov K.A., Schelle B., Bayer S., RA Scheiner B., Weinand H., Weissbrich B., Ziebuhr J.; RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. RN [9] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate Shanghai QXC1; RA Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.; RT "Analysis of SARS coronavirus genome in Shanghai isolates."; RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases. RN [10] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate Taiwan TC1, Isolate Taiwan TC2, and Isolate Taiwan TC3; RA Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.; RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases. RN [11] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS, and Isolate TWY; RA Shu H.Y., Wu K.M., Tsai S.F.; RT "The complete genome of SARS coronavirus TWH."; RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases. RN [12] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate HSR 1; RA Canducci F., Clementi M., Poli G., Vicenzi E.; RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases. RN [13] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RC STRAIN=Isolate AS; RA Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M., RA Ruan Y.J., Salemi M.; RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases. RN [14] RP FUNCTION. RX PubMed=16107218; DOI=10.1186/1743-422x-2-66; RA Yuan X., Shan Y., Zhao Z., Chen J., Cong Y.; RT "G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected RT cells."; RL Virol. J. 2:66-66(2005). RN [15] RP SUBCELLULAR LOCATION. RX PubMed=16046244; DOI=10.1016/j.virusres.2005.06.001; RA Yuan X., Yao Z., Shan Y., Chen B., Yang Z., Wu J., Zhao Z., Chen J., RA Cong Y.; RT "Nucleolar localization of non-structural protein 3b, a protein RT specifically encoded by the severe acute respiratory syndrome RT coronavirus."; RL Virus Res. 114:70-79(2005). RN [16] RP FUNCTION, AND SUBCELLULAR LOCATION. RX PubMed=16682811; DOI=10.1016/s1016-8478(23)12878-0; RA Yuan X., Shan Y., Yao Z., Li J., Zhao Z., Chen J., Cong Y.; RT "Mitochondrial location of severe acute respiratory syndrome coronavirus 3b RT protein."; RL Mol. Cells 21:186-191(2006). RN [17] RP INTERACTION WITH HOST RUNX1. RX PubMed=22253733; DOI=10.1371/journal.pone.0029542; RA Varshney B., Agnihothram S., Agnihotram S., Tan Y.J., Baric R., Lal S.K.; RT "SARS coronavirus 3b accessory protein modulates transcriptional activity RT of RUNX1b."; RL PLoS ONE 7:E29542-E29542(2012). CC -!- FUNCTION: Induces host cell G0/G1 arrest and apoptosis. CC {ECO:0000269|PubMed:16107218, ECO:0000269|PubMed:16682811}. CC -!- SUBUNIT: Interacts with host RUNX1 isoform b. CC {ECO:0000269|PubMed:22253733}. CC -!- INTERACTION: CC P59633; Q01196-1: RUNX1; Xeno; NbExp=6; IntAct=EBI-25489004, EBI-925940; CC -!- SUBCELLULAR LOCATION: Host nucleus, host nucleolus CC {ECO:0000269|PubMed:16046244}. Host mitochondrion CC {ECO:0000269|PubMed:16682811}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY278741; AAP13447.1; -; Genomic_RNA. DR EMBL; AY274119; AAP41039.1; -; Genomic_RNA. DR EMBL; AY282752; -; NOT_ANNOTATED_CDS; Genomic_RNA. DR EMBL; AY278554; AAP13569.1; -; Genomic_RNA. DR EMBL; AY278491; -; NOT_ANNOTATED_CDS; Genomic_RNA. DR EMBL; AY278487; -; NOT_ANNOTATED_CDS; Genomic_RNA. DR EMBL; AY278488; AAP30032.1; -; Genomic_RNA. DR EMBL; AY278489; AAP51229.1; -; Genomic_RNA. DR EMBL; AY278490; -; NOT_ANNOTATED_CDS; Genomic_RNA. DR EMBL; AY279354; -; NOT_ANNOTATED_CDS; Genomic_RNA. DR EMBL; AY291451; AAP37019.1; -; Genomic_RNA. DR EMBL; AY310120; AAP50487.1; -; Genomic_RNA. DR EMBL; AY291315; AAP33699.1; -; Genomic_RNA. DR EMBL; AY463059; AAR86789.1; -; Genomic_RNA. DR EMBL; AY338174; AAQ01599.1; -; Genomic_RNA. DR EMBL; AY338175; AAQ01611.1; -; Genomic_RNA. DR EMBL; AY348314; AAP97884.1; -; Genomic_RNA. DR EMBL; AP006557; BAC81350.1; -; Genomic_RNA. DR EMBL; AP006558; BAC81364.1; -; Genomic_RNA. DR EMBL; AP006559; BAC81378.1; -; Genomic_RNA. DR EMBL; AP006560; BAC81392.1; -; Genomic_RNA. DR EMBL; AP006561; BAC81406.1; -; Genomic_RNA. DR EMBL; AY323977; AAP72976.1; -; Genomic_RNA. DR EMBL; AY427439; AAQ94062.1; -; Genomic_RNA. DR SMR; P59633; -. DR BioGRID; 4383918; 121. DR IntAct; P59633; 115. DR Reactome; R-HSA-9692916; SARS-CoV-1 activates/modulates innate immune responses. DR SIGNOR; P59633; -. DR Proteomes; UP000000354; Segment. DR Proteomes; UP000103670; Genome. DR Proteomes; UP000109640; Genome. DR Proteomes; UP000116947; Genome. DR Proteomes; UP000121636; Genome. DR Proteomes; UP000131569; Genome. DR Proteomes; UP000131955; Genome. DR Proteomes; UP000137377; Genome. DR Proteomes; UP000138690; Genome. DR Proteomes; UP000143093; Genome. DR Proteomes; UP000145651; Genome. DR Proteomes; UP000146108; Genome. DR Proteomes; UP000146181; Genome. DR Proteomes; UP000146296; Genome. DR Proteomes; UP000148194; Genome. DR Proteomes; UP000153467; Genome. DR Proteomes; UP000160648; Genome. DR Proteomes; UP000164441; Genome. DR Proteomes; UP000172416; Genome. DR GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell. DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell. DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW. DR GO; GO:0001817; P:regulation of cytokine production; IEP:CACAO. DR GO; GO:0052150; P:symbiont-mediated perturbation of host apoptosis; IEA:UniProtKB-KW. DR GO; GO:0039646; P:symbiont-mediated perturbation of host cell cycle G0/G1 transition checkpoint; IEA:UniProtKB-KW. DR CDD; cd21649; SARS-CoV_ORF3b; 1. DR InterPro; IPR022117; SARS_3b. DR Pfam; PF12383; SARS_3b; 1. PE 1: Evidence at protein level; KW Apoptosis; G0/G1 host cell cycle checkpoint dysregulation by virus; KW Host mitochondrion; Host nucleus; Host-virus interaction; KW Modulation of host cell apoptosis by virus; KW Modulation of host cell cycle by virus; Reference proteome. FT CHAIN 1..154 FT /note="ORF3b protein" FT /id="PRO_0000106132" FT REGION 80..138 FT /note="Mitochondrial targeting signal" FT REGION 134..154 FT /note="Nucleolar targeting" FT MOTIF 135..153 FT /note="Bipartite nuclear localization signal" FT /evidence="ECO:0000255" FT VARIANT 31 FT /note="K -> Q (in strain: Isolate GD01)" FT VARIANT 99 FT /note="T -> I (in strain: Isolate BJ02)" FT VARIANT 115 FT /note="L -> Q (in strain: Isolate GD01)" FT VARIANT 121 FT /note="Q -> P (in strain: Isolate BJ01 and Isolate BJ04)" SQ SEQUENCE 154 AA; 17750 MW; 82AB0B68F11060A8 CRC64; MMPTTLFAGT HITMTTVYHI TVSQIQLSLL KVTAFQHQNS KKTTKLVVIL RIGTQVLKTM SLYMAISPKF TTSLSLHKLL QTLVLKMLHS SSLTSLLKTH RMCKYTQSTA LQELLIQQWI QFMMSRRRLL ACLCKHKKVS TNLCTHSFRK KQVR //