ID NS7B_SARS2 Reviewed; 43 AA.
AC P0DTD8;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 22-APR-2020, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=ORF7b protein;
DE Short=ORF7b;
DE AltName: Full=Accessory protein 7b;
GN ORFNames=7b;
OS Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Betacoronavirus; Sarbecovirus;
OC Severe acute respiratory syndrome coronavirus.
OX NCBI_TaxID=2697049;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=32015508; DOI=10.1038/s41586-020-2008-3;
RA Wu F., Zhao S., Yu B., Chen Y.-M., Wang W., Song Z.-G., Hu Y., Tao Z.-W.,
RA Tian J.-H., Pei Y.-Y., Yuan M.-L., Zhang Y.-L., Dai F.-H., Liu Y.,
RA Wang Q.-M., Zheng J.-J., Xu L., Holmes E.C., Zhang Y.-Z.;
RT "A new coronavirus associated with human respiratory disease in China.";
RL Nature 579:265-269(2020).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=33060197; DOI=10.1126/science.abe9403;
RG QCRG Structural Biology Consortium;
RG Zoonomia Consortium;
RA Gordon D.E., Hiatt J., Bouhaddou M., Rezelj V.V., Ulferts S., Braberg H.,
RA Jureka A.S., Obernier K., Guo J.Z., Batra J., Kaake R.M., Weckstein A.R.,
RA Owens T.W., Gupta M., Pourmal S., Titus E.W., Cakir M., Soucheray M.,
RA McGregor M., Cakir Z., Jang G., O'Meara M.J., Tummino T.A., Zhang Z.,
RA Foussard H., Rojc A., Zhou Y., Kuchenov D., Huettenhain R., Xu J.,
RA Eckhardt M., Swaney D.L., Fabius J.M., Ummadi M., Tutuncuoglu B.,
RA Rathore U., Modak M., Haas P., Haas K.M., Naing Z.Z.C., Pulido E.H.,
RA Shi Y., Barrio-Hernandez I., Memon D., Petsalaki E., Dunham A.,
RA Marrero M.C., Burke D., Koh C., Vallet T., Silvas J.A., Azumaya C.M.,
RA Billesboelle C., Brilot A.F., Campbell M.G., Diallo A., Dickinson M.S.,
RA Diwanji D., Herrera N., Hoppe N., Kratochvil H.T., Liu Y., Merz G.E.,
RA Moritz M., Nguyen H.C., Nowotny C., Puchades C., Rizo A.N.,
RA Schulze-Gahmen U., Smith A.M., Sun M., Young I.D., Zhao J., Asarnow D.,
RA Biel J., Bowen A., Braxton J.R., Chen J., Chio C.M., Chio U.S.,
RA Deshpande I., Doan L., Faust B., Flores S., Jin M., Kim K., Lam V.L.,
RA Li F., Li J., Li Y.L., Li Y., Liu X., Lo M., Lopez K.E., Melo A.A.,
RA Moss F.R. III, Nguyen P., Paulino J., Pawar K.I., Peters J.K.,
RA Pospiech T.H. Jr., Safari M., Sangwan S., Schaefer K., Thomas P.V.,
RA Thwin A.C., Trenker R., Tse E., Tsui T.K.M., Wang F., Whitis N., Yu Z.,
RA Zhang K., Zhang Y., Zhou F., Saltzberg D., Hodder A.J., Shun-Shion A.S.,
RA Williams D.M., White K.M., Rosales R., Kehrer T., Miorin L., Moreno E.,
RA Patel A.H., Rihn S., Khalid M.M., Vallejo-Gracia A., Fozouni P.,
RA Simoneau C.R., Roth T.L., Wu D., Karim M.A., Ghoussaini M., Dunham I.,
RA Berardi F., Weigang S., Chazal M., Park J., Logue J., McGrath M.,
RA Weston S., Haupt R., Hastie C.J., Elliott M., Brown F., Burness K.A.,
RA Reid E., Dorward M., Johnson C., Wilkinson S.G., Geyer A., Giesel D.M.,
RA Baillie C., Raggett S., Leech H., Toth R., Goodman N., Keough K.C.,
RA Lind A.L., Klesh R.J., Hemphill K.R., Carlson-Stevermer J., Oki J.,
RA Holden K., Maures T., Pollard K.S., Sali A., Agard D.A., Cheng Y.,
RA Fraser J.S., Frost A., Jura N., Kortemme T., Manglik A., Southworth D.R.,
RA Stroud R.M., Alessi D.R., Davies P., Frieman M.B., Ideker T., Abate C.,
RA Jouvenet N., Kochs G., Shoichet B., Ott M., Palmarini M., Shokat K.M.,
RA Garcia-Sastre A., Rassen J.A., Grosse R., Rosenberg O.S., Verba K.A.,
RA Basler C.F., Vignuzzi M., Peden A.A., Beltrao P., Krogan N.J.;
RT "Comparative host-coronavirus protein interaction networks reveal pan-viral
RT disease mechanisms.";
RL Science 0:0-0(2020).
CC -!- INTERACTION:
CC P0DTD8; Q7Z434: MAVS; Xeno; NbExp=3; IntAct=EBI-25475914, EBI-995373;
CC P0DTD8; P60468: SEC61B; Xeno; NbExp=3; IntAct=EBI-25475914, EBI-1788819;
CC P0DTD8; Q9NPR2: SEMA4B; Xeno; NbExp=3; IntAct=EBI-25475914, EBI-25678971;
CC P0DTD8; O43765: SGTA; Xeno; NbExp=3; IntAct=EBI-25475914, EBI-347996;
CC P0DTD8; P46977: STT3A; Xeno; NbExp=3; IntAct=EBI-25475914, EBI-719212;
CC P0DTD8; Q9NUH8: TMEM14B; Xeno; NbExp=3; IntAct=EBI-25475914, EBI-8638294;
CC P0DTD8; Q9NWC5: TMEM45A; Xeno; NbExp=3; IntAct=EBI-25475914, EBI-10823938;
CC P0DTD8; Q9H1C4: UNC93B1; Xeno; NbExp=2; IntAct=EBI-25475914, EBI-4401271;
CC -!- SUBCELLULAR LOCATION: Host Golgi apparatus membrane
CC {ECO:0000269|PubMed:33060197}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:Q7TFA1}. Host endosome membrane
CC {ECO:0000269|PubMed:33060197}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:Q7TFA1}.
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DR EMBL; MN908947; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR BioGRID; 4383871; 1728.
DR IntAct; P0DTD8; 481.
DR MINT; P0DTD8; -.
DR AGR; RefSeq:YP_009725318; -.
DR PRO; PR:P0DTD8; -.
DR Proteomes; UP000464024; Genome.
DR GO; GO:0044175; C:host cell endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR CDD; cd21623; ORF7b_SARS-CoV-2; 1.
DR InterPro; IPR021532; NS7B_bCoV.
DR Pfam; PF11395; bCoV_NS7B; 1.
PE 1: Evidence at protein level;
KW Host endosome; Host Golgi apparatus; Host membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..43
FT /note="ORF7b protein"
FT /id="PRO_0000449799"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 11
FT /note="L -> F (in strain: Omicron/BA.4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 43 AA; 5180 MW; 33DE6E3EBAEF1D9F CRC64;
MIELSLIDFY LCFLAFLLFL VLIMLIIFWF SLELQDHNET CHA
//