ID   NS8_SARS2               Reviewed;         121 AA.
AC   P0DTC8;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   22-APR-2020, sequence version 1.
DT   10-FEB-2021, entry version 6.
DE   RecName: Full=ORF8 protein;
DE            Short=ORF8;
DE   AltName: Full=Non-structural protein 8;
DE            Short=ns8;
DE   Flags: Precursor;
GN   ORFNames=8;
OS   Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Sarbecovirus.
OX   NCBI_TaxID=2697049;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=32015508; DOI=10.1038/s41586-020-2008-3;
RA   Wu F., Zhao S., Yu B., Chen Y.-M., Wang W., Song Z.-G., Hu Y., Tao Z.-W.,
RA   Tian J.-H., Pei Y.-Y., Yuan M.-L., Zhang Y.-L., Dai F.-H., Liu Y.,
RA   Wang Q.-M., Zheng J.-J., Xu L., Holmes E.C., Zhang Y.-Z.;
RT   "A new coronavirus associated with human respiratory disease in China.";
RL   Nature 579:265-269(2020).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=isolate 2019-nCoV_HKU-SZ-002a_2020;
RX   PubMed=31986261; DOI=10.1016/s0140-6736(20)30154-9;
RA   Chan J.F., Yuan S., Kok K.H., To K.K., Chu H., Yang J., Xing F., Liu J.,
RA   Yip C.C., Poon R.W., Tsoi H.W., Lo S.K., Chan K.H., Poon V.K., Chan W.M.,
RA   Ip J.D., Cai J.P., Cheng V.C., Chen H., Hui C.K., Yuen K.Y.;
RT   "A familial cluster of pneumonia associated with the 2019 novel coronavirus
RT   indicating person-to-person transmission: a study of a family cluster.";
RL   Lancet 395:514-523(2020).
RN   [3]
RP   MISCELLANEOUS.
RX   PubMed=32854725; DOI=10.1186/s12985-020-01402-1;
RA   Michel C.J., Mayer C., Poch O., Thompson J.D.;
RT   "Characterization of accessory genes in coronavirus genomes.";
RL   Virol. J. 17:131-131(2020).
RN   [4]
RP   MISCELLANEOUS.
RX   PubMed=32807944; DOI=10.1038/s41590-020-0773-7;
RA   Hachim A., Kavian N., Cohen C.A., Chin A.W.H., Chu D.K.W., Mok C.K.P.,
RA   Tsang O.T.Y., Yeung Y.C., Perera R.A.P.M., Poon L.L.M., Peiris J.S.M.,
RA   Valkenburg S.A.;
RT   "ORF8 and ORF3b antibodies are accurate serological markers of early and
RT   late SARS-CoV-2 infection.";
RL   Nat. Immunol. 0:0-0(2020).
RN   [5]
RP   INTERACTION WITH HUMAN IL17RA, AND SUBCELLULAR LOCATION.
RX   PubMed=33060197; DOI=10.1126/science.abe9403;
RG   QCRG Structural Biology Consortium;
RG   Zoonomia Consortium;
RA   Gordon D.E., Hiatt J., Bouhaddou M., Rezelj V.V., Ulferts S., Braberg H.,
RA   Jureka A.S., Obernier K., Guo J.Z., Batra J., Kaake R.M., Weckstein A.R.,
RA   Owens T.W., Gupta M., Pourmal S., Titus E.W., Cakir M., Soucheray M.,
RA   McGregor M., Cakir Z., Jang G., O'Meara M.J., Tummino T.A., Zhang Z.,
RA   Foussard H., Rojc A., Zhou Y., Kuchenov D., Huettenhain R., Xu J.,
RA   Eckhardt M., Swaney D.L., Fabius J.M., Ummadi M., Tutuncuoglu B.,
RA   Rathore U., Modak M., Haas P., Haas K.M., Naing Z.Z.C., Pulido E.H.,
RA   Shi Y., Barrio-Hernandez I., Memon D., Petsalaki E., Dunham A.,
RA   Marrero M.C., Burke D., Koh C., Vallet T., Silvas J.A., Azumaya C.M.,
RA   Billesboelle C., Brilot A.F., Campbell M.G., Diallo A., Dickinson M.S.,
RA   Diwanji D., Herrera N., Hoppe N., Kratochvil H.T., Liu Y., Merz G.E.,
RA   Moritz M., Nguyen H.C., Nowotny C., Puchades C., Rizo A.N.,
RA   Schulze-Gahmen U., Smith A.M., Sun M., Young I.D., Zhao J., Asarnow D.,
RA   Biel J., Bowen A., Braxton J.R., Chen J., Chio C.M., Chio U.S.,
RA   Deshpande I., Doan L., Faust B., Flores S., Jin M., Kim K., Lam V.L.,
RA   Li F., Li J., Li Y.L., Li Y., Liu X., Lo M., Lopez K.E., Melo A.A.,
RA   Moss F.R. III, Nguyen P., Paulino J., Pawar K.I., Peters J.K.,
RA   Pospiech T.H. Jr., Safari M., Sangwan S., Schaefer K., Thomas P.V.,
RA   Thwin A.C., Trenker R., Tse E., Tsui T.K.M., Wang F., Whitis N., Yu Z.,
RA   Zhang K., Zhang Y., Zhou F., Saltzberg D., Hodder A.J., Shun-Shion A.S.,
RA   Williams D.M., White K.M., Rosales R., Kehrer T., Miorin L., Moreno E.,
RA   Patel A.H., Rihn S., Khalid M.M., Vallejo-Gracia A., Fozouni P.,
RA   Simoneau C.R., Roth T.L., Wu D., Karim M.A., Ghoussaini M., Dunham I.,
RA   Berardi F., Weigang S., Chazal M., Park J., Logue J., McGrath M.,
RA   Weston S., Haupt R., Hastie C.J., Elliott M., Brown F., Burness K.A.,
RA   Reid E., Dorward M., Johnson C., Wilkinson S.G., Geyer A., Giesel D.M.,
RA   Baillie C., Raggett S., Leech H., Toth R., Goodman N., Keough K.C.,
RA   Lind A.L., Klesh R.J., Hemphill K.R., Carlson-Stevermer J., Oki J.,
RA   Holden K., Maures T., Pollard K.S., Sali A., Agard D.A., Cheng Y.,
RA   Fraser J.S., Frost A., Jura N., Kortemme T., Manglik A., Southworth D.R.,
RA   Stroud R.M., Alessi D.R., Davies P., Frieman M.B., Ideker T., Abate C.,
RA   Jouvenet N., Kochs G., Shoichet B., Ott M., Palmarini M., Shokat K.M.,
RA   Garcia-Sastre A., Rassen J.A., Grosse R., Rosenberg O.S., Verba K.A.,
RA   Basler C.F., Vignuzzi M., Peden A.A., Beltrao P., Krogan N.J.;
RT   "Comparative host-coronavirus protein interaction networks reveal pan-viral
RT   disease mechanisms.";
RL   Science 0:0-0(2020).
CC   -!- FUNCTION: May play a role in modulating host immune response
CC       (Probable). May play a role in blocking host IL17 cytokine by its
CC       interaction with host IL17RA (PubMed:31986261).
CC       {ECO:0000303|PubMed:31986261, ECO:0000305}.
CC   -!- SUBUNIT: May interact with host IL17RA. {ECO:0000269|PubMed:33060197}.
CC   -!- INTERACTION:
CC       P0DTC8; P0DTC8: 8; NbExp=2; IntAct=EBI-25475900, EBI-25475900;
CC       P0DTC8; P04439: HLA-A; Xeno; NbExp=3; IntAct=EBI-25475900, EBI-1042870;
CC       P0DTC8; Q96F46: IL17RA; Xeno; NbExp=3; IntAct=EBI-25475900, EBI-5591258;
CC   -!- SUBCELLULAR LOCATION: Host endosome {ECO:0000269|PubMed:33060197}.
CC       Note=Localizes to host endosome when expressed in cell culture.
CC       {ECO:0000269|PubMed:33060197}.
CC   -!- MISCELLANEOUS: Similar to some Bat coronavirus ns8 genes, but is
CC       entirely different from SARS ns8a or Ns8b (Probable). Elicits strong
CC       specific antibody response (PubMed:32807944).
CC       {ECO:0000269|PubMed:32807944, ECO:0000305|PubMed:32854725}.
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DR   EMBL; MN908947; QHD43422.1; -; Genomic_RNA.
DR   EMBL; MN938384; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   RefSeq; YP_009724396.1; NC_045512.2.
DR   PDB; 7JTL; X-ray; 2.04 A; A/B=18-121.
DR   PDB; 7JX6; X-ray; 1.61 A; A/B=18-121.
DR   PDBsum; 7JTL; -.
DR   PDBsum; 7JX6; -.
DR   SMR; P0DTC8; -.
DR   BioGRID; 4383873; 794.
DR   ComplexPortal; CPX-6147; SARS-CoV-2 ORF8 complex.
DR   IntAct; P0DTC8; 117.
DR   GeneID; 43740577; -.
DR   KEGG; vg:43740577; -.
DR   PRO; PR:P0DTC8; -.
DR   Proteomes; UP000464024; Genome.
DR   InterPro; IPR022722; NS8_bCoV.
DR   Pfam; PF12093; bCoV_NS8; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Host endosome; Reference proteome; Signal.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..121
FT                   /note="ORF8 protein"
FT                   /id="PRO_0000449655"
FT   VARIANT         84
FT                   /note="L -> S (in isolate 2019-nCoV_HKU-SZ-002a_2020)"
SQ   SEQUENCE   121 AA;  13831 MW;  912AD9E147A64907 CRC64;
     MKFLVFLGII TTVAAFHQEC SLQSCTQHQP YVVDDPCPIH FYSKWYIRVG ARKSAPLIEL
     CVDEAGSKSP IQYIDIGNYT VSCLPFTINC QEPKLGSLVV RCSFYEDFLE YHDVRVVLDF
     I
//