ID   VEMP_SARS2              Reviewed;          75 AA.
AC   P0DTC4;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   22-APR-2020, sequence version 1.
DT   10-FEB-2021, entry version 6.
DE   RecName: Full=Envelope small membrane protein {ECO:0000255|HAMAP-Rule:MF_04204};
DE            Short=E;
DE            Short=sM protein {ECO:0000255|HAMAP-Rule:MF_04204};
GN   Name=E {ECO:0000255|HAMAP-Rule:MF_04204}; ORFNames=4;
OS   Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Sarbecovirus.
OX   NCBI_TaxID=2697049;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RX   PubMed=32015508; DOI=10.1038/s41586-020-2008-3;
RA   Wu F., Zhao S., Yu B., Chen Y.-M., Wang W., Song Z.-G., Hu Y., Tao Z.-W.,
RA   Tian J.-H., Pei Y.-Y., Yuan M.-L., Zhang Y.-L., Dai F.-H., Liu Y.,
RA   Wang Q.-M., Zheng J.-J., Xu L., Holmes E.C., Zhang Y.-Z.;
RT   "A new coronavirus associated with human respiratory disease in China.";
RL   Nature 579:265-269(2020).
CC   -!- FUNCTION: Plays a central role in virus morphogenesis and assembly.
CC       Acts as a viroporin and self-assembles in host membranes forming
CC       pentameric protein-lipid pores that allow ion transport. Also plays a
CC       role in the induction of apoptosis. {ECO:0000255|HAMAP-Rule:MF_04204}.
CC   -!- SUBUNIT: Homopentamer. Interacts with membrane protein M in the budding
CC       compartment of the host cell, which is located between endoplasmic
CC       reticulum and the Golgi complex. Interacts with Nucleoprotein (By
CC       similarity). Interacts with the accessory proteins 3a and 7a. Interacts
CC       (via C-terminus) with human MPP5 (via PDZ domain); this inhibits the
CC       interaction between human MPP5 and human CRB3, and causes delayed tight
CC       junction formation and defective cell polarity (By similarity).
CC       {ECO:0000250|UniProtKB:P59637, ECO:0000255|HAMAP-Rule:MF_04204}.
CC   -!- SUBCELLULAR LOCATION: Host Golgi apparatus membrane {ECO:0000255|HAMAP-
CC       Rule:MF_04204}; Single-pass type III membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_04204}. Note=The cytoplasmic tail functions
CC       as a Golgi complex-targeting signal. {ECO:0000255|HAMAP-Rule:MF_04204}.
CC   -!- SIMILARITY: Belongs to the betacoronaviruses E protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04204}.
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DR   EMBL; MN908947; QHD43418.1; -; Genomic_RNA.
DR   RefSeq; YP_009724392.1; NC_045512.2.
DR   PDB; 7K3G; NMR; -; A/B/C/D/E=8-38.
DR   PDBsum; 7K3G; -.
DR   BMRB; P0DTC4; -.
DR   SMR; P0DTC4; -.
DR   BioGRID; 4383845; 855.
DR   IntAct; P0DTC4; 17.
DR   ABCD; P0DTC4; 9 sequenced antibodies.
DR   GeneID; 43740570; -.
DR   KEGG; vg:43740570; -.
DR   Reactome; R-HSA-9694322; Virion Assembly and Release.
DR   Reactome; R-HSA-9694493; Maturation of protein E.
DR   Reactome; R-HSA-9694614; Attachment and Entry.
DR   Reactome; R-HSA-9694635; Translation of structural proteins.
DR   PRO; PR:P0DTC4; -.
DR   Proteomes; UP000464024; Genome.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0039707; P:pore formation by virus in membrane of host cell; IEA:InterPro.
DR   GO; GO:0046760; P:viral budding from Golgi membrane; IEA:InterPro.
DR   InterPro; IPR003873; E_protein_CoV.
DR   Pfam; PF02723; CoV_E; 1.
DR   PROSITE; PS51926; COV_E; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Apoptosis; Host Golgi apparatus; Host membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..75
FT                   /note="Envelope small membrane protein"
FT                   /id="PRO_0000449651"
FT   TOPO_DOM        1..16
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04204"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04204"
FT   TOPO_DOM        38..75
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04204"
FT   MOTIF           72..75
FT                   /note="PDZ-binding; required for interaction with human
FT                   MPP5"
FT                   /evidence="ECO:0000250|UniProtKB:P59637"
SQ   SEQUENCE   75 AA;  8365 MW;  5C431BD2AA1B6B99 CRC64;