Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 S 1 -1 0 -1 -1 0 0 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.60 0.06 2 K -1 3 1 -1 -4 1 0 -2 -1 -3 -3 5 -2 -4 -2 0 -1 -3 -2 -3 0 10 7 0 0 0 0 0 0 0 0 82 0 0 0 0 0 0 0 0 0.58 0.05 3 M -2 -2 -4 -4 -2 -2 -3 -4 -3 1 4 -3 4 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 82 0 18 0 0 0 0 0 0 0 0.50 0.04 4 S 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -1 2 5 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 22 78 0 0 0 0.52 0.05 5 D -2 -2 1 6 -4 0 2 -2 -1 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 92 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 1.02 0.05 6 V -1 -3 -3 -4 -1 -2 -3 -4 -3 2 3 -3 2 0 -3 -2 -1 -2 -1 3 0 0 0 0 0 0 0 0 0 6 42 0 8 0 0 0 0 0 0 43 0.35 0.03 7 K -1 2 -1 -1 -4 1 1 -2 -1 -3 -3 6 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.69 0.06 8 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.62 0.08 9 T 1 -2 -1 -2 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -3 -2 1 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 73 0 0 10 0.41 0.04 10 S 0 -1 5 0 -2 0 -1 -1 0 -3 -3 0 -2 -3 -2 3 2 -4 -2 -2 0 0 53 0 0 0 0 0 0 0 0 0 0 0 0 35 11 0 0 0 0.51 0.04 11 V 0 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.50 0.05 12 V 0 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.50 0.05 13 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.57 0.04 14 L -2 -2 -4 -4 -2 -2 -3 -4 -3 1 4 -3 4 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 82 0 18 0 0 0 0 0 0 0 0.50 0.04 15 S 0 -1 3 0 -2 1 -1 3 -1 -3 -3 -1 -2 -3 -2 2 1 -3 -2 -3 0 0 21 0 0 11 0 33 0 0 0 0 0 0 0 30 6 0 0 0 0.39 0.02 16 V -1 -3 -3 -4 7 -3 -3 -3 -3 2 1 -3 0 -1 -3 -2 -1 -3 -2 2 0 0 0 0 38 0 0 0 0 6 20 0 0 0 0 0 0 0 0 35 0.50 0.03 17 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.57 0.04 18 Q 0 0 0 -1 -2 5 1 -2 0 -3 -3 0 -1 -3 -1 2 1 -3 -2 -2 0 0 0 0 0 56 0 0 0 0 0 0 0 0 0 33 11 0 0 0 0.47 0.04 19 Q -1 1 2 0 -3 4 1 -2 4 -3 -3 2 -1 -3 -2 1 -1 -3 -1 -3 0 0 12 0 0 35 0 0 21 0 0 20 0 0 0 12 0 0 0 0 0.40 0.03 20 L -2 -2 -3 -4 -2 -2 -3 -4 -3 1 4 -2 5 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 67 0 33 0 0 0 0 0 0 0 0.47 0.04 21 R -2 2 4 0 -3 0 0 -2 6 -4 -3 0 -2 -2 -3 -1 -1 -3 0 -3 0 18 36 0 0 0 0 0 46 0 0 0 0 0 0 0 0 0 0 0 0.62 0.04 22 V -1 -3 -3 -4 -1 -3 -3 -4 -4 3 2 -3 1 -1 -3 -2 -1 -3 -1 4 0 0 0 0 0 0 0 0 0 28 17 0 0 0 0 0 0 0 0 54 0.38 0.03 23 E 2 -1 -1 0 -2 1 4 -1 -1 -3 -3 0 -2 -3 -1 1 -1 -3 -2 -2 39 0 0 0 0 0 55 0 0 0 0 0 0 0 0 6 0 0 0 0 0.44 0.04 24 S 3 -1 0 -1 -1 -1 -1 0 -1 -2 -2 -1 -2 -3 -1 4 1 -3 -2 -1 41 0 0 0 0 0 0 0 0 0 0 0 0 0 0 59 0 0 0 0 0.43 0.04 25 S -1 -1 6 1 -2 0 -1 -1 0 -3 -4 0 -2 -3 -2 2 0 -4 -2 -3 0 0 72 0 0 0 0 0 0 0 0 0 0 0 0 28 0 0 0 0 0.66 0.05 26 S 1 -1 0 -1 -1 0 0 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.60 0.06 27 K -1 3 0 -1 -3 1 0 -2 -1 -3 -3 5 -2 -3 -1 0 0 -3 -2 -2 0 12 0 0 0 0 0 0 0 0 0 76 0 0 0 6 6 0 0 0 0.51 0.04 28 L 1 -1 -2 -1 -2 0 3 -2 -2 0 2 -1 2 -1 -2 -1 -1 -2 -2 0 17 0 0 0 0 0 33 0 0 0 39 0 11 0 0 0 0 0 0 0 0.18 0.02 29 W -3 -3 -3 -4 -3 -2 -3 -3 3 -3 -2 -3 -2 0 -4 -3 -3 11 2 -4 0 0 0 0 0 0 0 0 11 0 0 0 0 0 0 0 0 89 0 0 2.33 0.09 30 A 4 -1 1 -1 -1 2 0 0 1 -2 -2 0 -1 -3 -1 1 0 -3 -2 -1 65 0 8 0 0 16 0 0 5 0 0 0 0 0 0 6 0 0 0 0 0.31 0.03 31 Q -2 -2 -2 -3 -3 1 -2 -3 2 -1 0 -2 -1 3 -3 -2 -2 1 7 -2 0 0 0 0 0 11 0 0 5 0 7 0 0 6 0 0 0 0 70 0 0.69 0.05 32 C -1 -4 -3 -4 10 -3 -4 -3 -4 -1 0 -4 -1 -2 -3 -1 -1 -3 -3 -1 0 0 0 0 89 0 0 0 0 0 11 0 0 0 0 0 0 0 0 0 1.32 0.07 33 V 0 -2 -2 -3 -1 -2 -2 -3 -3 2 0 -2 0 -1 -2 1 0 -3 -2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 21 0 0 0 79 0.31 0.04 34 Q -1 0 0 2 -3 2 2 0 -1 -2 -2 2 -2 -3 -2 0 1 -3 -2 -1 0 0 0 18 0 11 18 8 0 0 0 24 0 0 0 0 15 0 0 5 0.27 0.02 35 L -2 -3 -4 -4 2 -2 -3 -4 -3 1 4 -3 3 0 -3 -3 -1 -2 -1 1 0 0 0 0 6 0 0 0 0 0 87 0 6 0 0 0 0 0 0 0 0.49 0.04 36 H -2 -1 0 -2 -4 0 -1 -3 9 -4 -3 -1 -2 -2 -3 -1 -2 -3 1 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 1.24 0.08 37 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.95 0.06 38 D -1 1 0 3 -4 1 4 -2 -1 -3 -3 3 -2 -4 -1 0 -1 -4 -3 -3 0 0 0 22 0 0 42 0 0 0 0 36 0 0 0 0 0 0 0 0 0.54 0.03 39 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.56 0.05 40 L -2 -2 3 -1 -2 -2 -2 -3 -2 1 3 -2 1 -1 -3 -1 -1 -3 -2 0 0 0 33 0 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0 0.30 0.04 41 L 3 -2 -2 -3 -1 -2 -2 -1 -2 0 2 -2 1 -1 -2 0 -1 -2 -2 0 51 0 0 0 0 0 0 0 0 0 44 0 0 0 0 5 0 0 0 0 0.24 0.03 42 A 2 -2 -1 -2 6 -1 -2 -1 -2 -2 -2 -1 -1 -3 -2 2 2 -3 -2 -1 28 0 0 0 33 0 0 0 0 0 0 0 0 0 0 19 21 0 0 0 0.43 0.03 43 K -1 -1 3 4 -2 0 0 -1 -1 -2 -3 1 -2 -3 -2 2 1 -4 -2 -1 0 0 19 32 0 0 0 0 0 0 0 11 0 0 0 21 11 0 0 6 0.34 0.02 44 D -2 -2 1 6 -4 -1 1 -2 -1 -4 -4 -1 -3 -4 -2 0 -1 -5 -3 -4 0 0 0 93 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 1.00 0.05 45 T 0 -2 -2 -2 -2 -2 -2 -3 -3 0 1 -2 0 -2 6 -1 1 -3 -2 0 6 0 0 0 0 0 0 0 0 0 21 0 0 0 51 0 11 0 0 11 0.81 0.03 46 T 0 -1 0 0 -2 0 3 1 -1 -2 -3 0 -2 -3 -1 2 3 -3 -2 -2 0 0 0 0 0 0 33 11 0 0 0 0 0 0 0 27 29 0 0 0 0.33 0.03 47 E 0 0 -1 0 -2 1 4 -2 -1 0 -1 1 -1 -2 -2 0 0 -3 -2 1 6 0 0 0 0 0 47 0 0 8 0 12 0 0 0 0 6 0 0 21 0.24 0.02 48 A 4 -2 -2 -2 4 -1 -2 0 -2 -1 -1 -1 -1 -2 -1 1 0 -3 -2 0 80 0 0 0 11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0.41 0.04 49 F -2 -1 -2 -3 -3 2 -1 -3 -1 0 1 -1 2 5 -3 -2 -2 0 2 -1 0 0 0 0 0 24 0 0 0 0 8 0 11 56 0 0 0 0 0 0 0.40 0.04 50 E -1 -1 0 4 -4 1 5 -2 -1 -4 -4 0 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 32 0 0 62 0 0 0 0 0 0 0 0 6 0 0 0 0 0.70 0.04 51 K 0 2 2 -1 -3 0 0 -2 -1 -1 0 3 2 -2 -2 0 -1 -3 -2 -1 7 6 17 0 0 0 0 0 0 0 6 46 18 0 0 0 0 0 0 0 0.22 0.03 52 M -2 -3 -4 -4 -2 -2 -3 -4 -3 1 4 -3 3 3 -3 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 65 0 11 24 0 0 0 0 0 0 0.43 0.03 53 V 1 -2 -3 -3 -1 -2 -3 -3 -3 2 3 -2 1 -1 -2 -1 -1 -3 -1 2 21 0 0 0 0 0 0 0 0 0 44 0 0 0 0 0 0 0 0 35 0.25 0.02 54 S 2 -1 -1 -1 3 2 0 1 -1 -2 -2 0 -1 -3 -1 2 0 -3 -2 -1 33 0 0 0 11 21 0 11 0 0 0 0 0 0 0 24 0 0 0 0 0.28 0.02 55 L -2 -2 -4 -4 -2 -2 -3 -4 -3 1 4 -3 3 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 89 0 11 0 0 0 0 0 0 0 0.52 0.04 56 L -2 -3 -4 -4 -2 -3 -3 -4 -3 2 4 -3 2 3 -3 -3 -2 -1 0 1 0 0 0 0 0 0 0 0 0 8 75 0 0 17 0 0 0 0 0 0 0.45 0.03 57 S 3 -2 -2 -2 -1 -1 -1 -1 -2 2 -1 -1 0 -2 -2 2 0 -3 -2 1 50 0 0 0 0 0 0 0 0 22 0 0 0 0 0 18 0 0 0 10 0.22 0.03 58 V -1 -2 -2 -3 -2 -2 -2 -3 -2 1 0 -2 0 4 -3 -1 2 -1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 33 0 0 29 0 0 39 0.27 0.03 59 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 4 -3 1 4 -4 -3 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 67 0 0 33 0 0 0 0 0 0 0.48 0.03 60 L -2 -3 -3 -4 -2 -2 -3 -4 -2 1 3 -3 3 4 -3 -3 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 51 0 17 32 0 0 0 0 0 0 0.42 0.03 61 S 2 -1 0 -1 4 -1 -1 -1 -2 -2 -2 -1 -2 -3 -1 4 1 -3 -2 -1 15 0 0 0 11 0 0 0 0 0 0 0 0 0 0 67 6 0 0 0 0.41 0.04 62 M -1 0 2 -1 -3 0 -1 -2 -1 0 0 2 2 2 -2 -1 -1 -2 0 -1 0 0 21 0 0 0 0 0 0 11 7 33 11 17 0 0 0 0 0 0 0.12 0.02 63 Q -1 -1 2 2 -3 1 0 -2 4 -3 -3 -1 -2 -3 4 1 -1 -3 -1 -3 0 0 12 11 0 11 0 0 21 0 0 0 0 0 28 17 0 0 0 0 0.53 0.03 64 G 2 -1 1 -1 -1 -1 -1 3 -1 -3 -3 -1 -2 -3 -1 3 0 -3 -2 -2 32 0 8 0 0 0 0 24 0 0 0 0 0 0 0 36 0 0 0 0 0.36 0.02 65 A 3 -1 2 2 -1 -1 -1 -1 -1 -2 -2 -1 -2 -3 -1 1 2 -3 -2 -1 51 0 17 12 0 0 0 0 0 0 0 0 0 0 0 0 20 0 0 0 0.28 0.03 66 V -1 -3 -3 -4 3 -3 -3 -4 -3 3 1 -3 0 3 -3 -2 -1 -2 0 3 0 0 0 0 8 0 0 0 0 19 0 0 0 24 0 0 0 0 0 49 0.32 0.03 67 D -1 -2 2 6 -4 -1 1 3 -1 -4 -4 -1 -3 -4 -2 0 -1 -4 -3 -4 0 0 7 68 0 0 0 24 0 0 0 0 0 0 0 0 0 0 0 0 0.74 0.04 68 I -1 -3 -3 -3 -1 -2 -3 -4 -3 3 4 -3 2 0 -3 -1 -1 -2 -1 2 0 0 0 0 0 0 0 0 0 24 63 0 0 0 0 7 0 0 0 6 0.36 0.03 69 N -1 -1 2 4 -3 0 2 2 -1 -4 -4 0 -3 -4 -2 1 -1 -4 -3 -3 0 0 12 44 0 0 12 18 0 0 0 7 0 0 0 8 0 0 0 0 0.49 0.03 70 K 1 0 -1 2 2 1 4 0 -1 -3 -3 1 -2 -3 -2 0 -1 -3 -2 -2 18 6 0 13 7 0 44 6 0 0 0 6 0 0 0 0 0 0 0 0 0.34 0.03 71 L -2 -3 -4 -4 -2 -2 -3 -4 -2 1 4 -3 2 1 -3 -3 -1 -1 2 1 0 0 0 0 0 0 0 0 0 0 76 0 0 0 0 0 0 0 11 12 0.43 0.04 72 C 2 -3 -3 -3 7 -2 -3 -2 -3 1 1 -2 0 -2 -2 0 -1 -3 -2 1 25 0 0 0 36 0 0 0 0 14 12 0 0 0 0 6 0 0 0 7 0.38 0.03 73 E -1 -1 1 4 -3 0 3 -2 -1 -3 -4 0 -3 -4 -2 1 -1 -4 -3 -3 0 0 6 42 0 0 33 0 0 0 0 0 0 0 0 19 0 0 0 0 0.58 0.04 74 E 0 -1 1 4 -3 0 2 -1 -1 -3 -3 0 -3 -3 -1 3 0 -4 -3 -3 0 0 0 42 0 0 19 0 0 0 0 0 0 0 0 39 0 0 0 0 0.53 0.04 75 M -2 -2 -3 -4 -2 -2 -3 -4 0 2 1 -2 2 2 -3 -2 -1 0 5 1 0 0 0 0 0 0 0 0 0 24 12 0 12 0 0 0 0 0 45 7 0.41 0.04 76 L -1 -3 -3 -3 -2 -3 -3 -3 -2 1 2 -3 1 5 -3 -1 -1 -1 1 0 6 0 0 0 0 0 0 0 0 0 36 0 0 45 0 7 0 0 0 6 0.34 0.03 77 D -1 0 2 4 -4 2 3 -2 -1 -3 -3 2 -2 -4 -2 0 -1 -4 -3 -3 0 0 12 37 0 6 27 0 0 0 0 17 0 0 0 0 0 0 0 0 0.54 0.03 78 N -2 1 5 4 -3 0 0 -1 2 -3 -4 0 -3 -3 -2 0 0 -4 -2 -3 0 11 45 32 0 0 0 0 6 0 0 0 0 0 0 0 6 0 0 0 0.54 0.04 79 R 0 1 3 1 -2 0 0 -1 -1 -3 -3 0 -2 -3 2 3 1 -4 -2 -2 0 13 22 7 0 0 0 0 0 0 0 0 0 0 13 37 8 0 0 0 0.36 0.03 80 A 2 -1 0 -1 -1 -1 -1 -1 -2 -2 -2 -1 -1 -3 -1 4 3 -3 -2 -1 13 0 0 0 0 0 0 0 0 0 0 0 0 0 0 52 35 0 0 0 0.41 0.04 81 T -1 -3 -2 -3 -1 -2 -2 -3 -3 3 1 -2 2 -1 -2 -1 2 -3 -2 4 0 0 0 0 0 0 0 0 0 15 0 0 7 0 0 0 19 0 0 59 0.30 0.03 82 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.59 0.05 83 Q -1 1 0 -1 -4 7 2 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.91 0.08 K Lambda Standard Ungapped 0.1270 0.3179 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1270 0.3179 PSI Gapped 0.0410 0.2670