Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -1 -2 -2 -3 -2 0 -2 -3 -2 1 2 -2 6 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.33 0.00 2 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.01 3 P -1 -1 -1 -1 -3 3 0 -2 -1 -3 -3 0 -2 -4 7 -1 -1 -3 -3 -3 0 0 0 0 0 34 0 0 0 0 0 0 0 0 66 0 0 0 0 0 1.23 0.00 4 P 1 -1 0 -1 -2 0 0 -1 -1 -3 -3 -1 -2 -3 5 3 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 33 67 0 0 0 0 0.65 0.01 5 C 0 -4 -3 -4 10 -3 -4 -3 -3 -1 -1 -3 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.19 0.00 6 Y -2 -2 -2 -3 -3 -2 -2 -3 2 -1 -1 -2 -1 3 -3 -2 -2 2 7 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0.85 0.00 7 N -2 0 6 1 -3 0 0 0 1 -4 -4 0 -2 -3 -2 1 0 -4 -2 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.72 0.00 8 F -2 -3 -3 -4 -3 -4 -4 -3 -1 0 0 -3 0 7 -4 -3 -2 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.73 0.00 9 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.01 10 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.51 0.00 11 E -1 0 0 2 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.00 12 Q -1 1 0 0 -3 6 2 -2 0 -3 -2 1 0 -4 -1 0 -1 -2 -2 -2 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.00 13 H -2 0 1 -1 -3 0 0 -2 8 -4 -3 -1 -2 -1 -2 -1 -2 -3 2 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0.85 0.00 14 C 0 -4 -3 -4 10 -3 -4 -3 -3 -1 -1 -3 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.19 0.00 15 Q -1 1 0 0 -3 6 2 -2 0 -3 -2 1 0 -4 -1 0 -1 -2 -2 -2 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.00 16 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.51 0.00 17 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.00 18 S 1 -1 1 0 -1 0 0 0 -1 -3 -3 0 -2 -3 -1 4 2 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.45 0.00 19 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 2 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.46 0.00 20 Q -1 1 0 0 -3 6 2 -2 0 -3 -2 1 0 -4 -1 0 -1 -2 -2 -2 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.00 21 R -1 4 0 -1 -4 1 1 -2 -1 -3 -3 4 -2 -3 -1 0 -1 -3 -2 -3 0 33 0 0 0 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0.52 0.01 22 E 0 -2 0 0 -3 0 2 5 -1 -4 -4 -1 -3 -4 -2 0 -1 -3 -3 -3 0 0 0 0 0 0 33 67 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.01 23 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.00 24 E -1 0 0 2 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.00 25 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.00 26 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.00 27 V 0 -3 -3 -4 -1 -2 -3 -4 -3 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.34 0.00 28 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.51 0.00 29 P -1 -2 -2 -2 -3 -1 -1 -2 -2 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.04 0.00 30 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.01 31 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.01 32 A 3 -2 -2 -2 -1 -1 -1 -1 -2 1 -1 -1 0 -2 -1 0 0 -3 -2 2 66 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 34 0.24 0.01 33 P -1 -2 -3 -2 -2 -2 -2 -3 -3 -1 1 -2 0 -2 7 -1 -1 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 33 0 0 0 67 0 0 0 0 0 1.14 0.00 34 H -2 0 1 -1 -3 0 0 -2 8 -4 -3 -1 -2 -1 -2 -1 -2 -3 2 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0.85 0.00 35 H -2 0 1 -1 -3 0 0 -2 8 -4 -3 -1 -2 -1 -2 -1 -2 -3 2 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0.85 0.00 36 V 0 -3 -3 -4 -1 -2 -3 -4 -3 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.34 0.00 37 V 0 -3 -3 -4 -1 -2 -3 -4 -3 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.34 0.00 38 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.00 39 V 0 -2 -2 -3 -1 -2 -2 -3 -3 2 0 -2 0 -1 -2 0 3 -3 -2 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 34 0 0 66 0.24 0.01 40 I -1 -3 -3 -4 -1 -3 -3 -4 -4 4 1 -3 1 0 -3 -2 -1 -3 -1 3 0 0 0 0 0 0 0 0 0 66 0 0 0 0 0 0 0 0 0 34 0.35 0.01 41 Q -1 1 0 0 -3 6 2 -2 0 -3 -2 1 0 -4 -1 0 -1 -2 -2 -2 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.00 42 E -1 0 0 2 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.00 43 I -1 -3 -4 -4 -1 -3 -4 -4 -4 4 2 -3 1 0 -3 -3 -1 -3 -1 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.38 0.00 44 Q -1 1 0 0 -3 6 2 -2 0 -3 -2 1 0 -4 -1 0 -1 -2 -2 -2 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.00 45 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.01 46 L -1 -1 -2 -2 -2 3 0 -3 -1 1 3 -1 2 0 -2 -2 -1 -2 -1 0 0 0 0 0 0 34 0 0 0 0 66 0 0 0 0 0 0 0 0 0 0.24 0.01 47 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.00 48 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.00 49 V 0 -3 -3 -4 -1 -2 -3 -4 -3 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.34 0.00 50 G 0 -3 0 -1 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 0 -2 -3 -3 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.04 0.00 51 E -1 0 0 2 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.00 52 I -2 -3 -4 -4 -1 -3 -3 -4 -3 4 3 -3 2 0 -3 -3 -1 -2 -1 2 0 0 0 0 0 0 0 0 0 66 34 0 0 0 0 0 0 0 0 0 0.36 0.01 53 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.01 54 L -1 -1 -2 -2 -2 3 0 -3 -1 1 3 -1 2 0 -3 -2 -1 -2 -1 0 0 0 0 0 0 33 0 0 0 0 67 0 0 0 0 0 0 0 0 0 0.25 0.01 55 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.01 56 E -1 0 0 2 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.00 57 W -3 -3 -4 -5 -3 -2 -3 -3 -3 -3 -2 -3 -2 1 -4 -3 -3 12 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.29 0.00 58 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.00 59 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.00 60 E -1 0 0 2 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.00 61 V 3 -2 -2 -3 -1 -2 -2 -1 -3 1 0 -2 0 -2 -2 0 0 -3 -2 3 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 50 0.21 0.00 62 V 0 -3 -3 -4 -1 -2 -3 -4 -3 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.34 0.00 63 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.51 0.00 64 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.00 65 P -1 -2 -2 -2 -3 -1 -1 -2 -2 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.03 0.00 66 S 1 -1 1 0 -1 0 0 0 -1 -3 -3 0 -2 -3 -1 4 2 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.45 0.00 67 R -2 6 0 -2 -4 1 0 -3 0 -3 -2 2 -2 -3 -2 -1 -1 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.74 0.00 68 Y -2 -2 -2 -3 -3 -2 -2 -3 2 -1 -1 -2 -1 3 -3 -2 -2 2 7 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0.85 0.00 69 C 0 -4 -3 -4 10 -3 -4 -3 -3 -1 -1 -3 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.19 0.00 70 C 0 -4 -3 -4 10 -3 -4 -3 -3 -1 -1 -3 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.19 0.00 K Lambda Standard Ungapped 0.1353 0.3242 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1353 0.3242 PSI Gapped 0.0410 0.2670