Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -1 -2 -3 -4 -2 -1 -2 -3 -2 1 2 -2 7 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.41 0.02 2 Y -2 -2 -3 -4 -2 -2 -3 -4 0 1 3 -3 1 2 -3 -2 -2 0 5 0 0 0 0 0 0 0 0 0 0 0 61 0 0 0 0 0 0 0 39 0 0.40 0.01 3 S 0 -2 -1 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -4 7 2 0 -4 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 61 39 0 0 0 0 1.12 0.01 4 F -3 -3 -3 -4 -3 -4 -4 -4 -1 0 0 -4 0 7 -4 -3 -2 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.78 0.01 5 V 0 -3 -3 -4 -1 -3 -3 -4 -4 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.37 0.01 6 S 0 0 0 -1 -2 4 1 -1 4 -3 -3 0 -1 -3 -1 2 0 -3 -1 -2 0 0 0 0 0 42 0 0 18 0 0 0 0 0 0 39 0 0 0 0 0.38 0.01 7 E -1 0 0 2 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.01 8 E -1 2 0 3 -4 4 3 -2 0 -4 -3 1 -2 -4 -2 0 -1 -3 -2 -3 0 16 0 26 0 29 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0.51 0.01 9 T -1 -2 -1 -2 -1 -2 -2 -3 -3 3 0 -2 0 -1 -2 0 4 -3 -2 2 0 0 0 0 0 0 0 0 0 45 0 0 0 0 0 0 55 0 0 0 0.28 0.02 10 G 2 -2 -1 -2 -2 -2 -2 6 -2 -3 -3 -2 -3 -3 -2 0 -1 -3 -3 -2 26 0 0 0 0 0 0 74 0 0 0 0 0 0 0 0 0 0 0 0 0.75 0.02 11 T 1 -2 -2 -2 -1 -1 -2 -2 -2 -1 0 -2 0 -1 -2 0 3 8 0 -1 16 0 0 0 0 0 0 0 0 0 16 0 0 0 0 0 42 26 0 0 0.47 0.02 12 L -2 -3 -3 -4 -2 -3 -3 -4 -1 1 2 -3 1 4 -4 -3 -2 1 4 0 0 0 0 0 0 0 0 0 0 13 28 0 0 32 0 0 0 0 26 0 0.41 0.01 13 I -1 -3 -4 -4 -1 -3 -4 -4 -4 4 1 -3 1 0 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 74 0 0 0 0 0 0 0 0 0 26 0.41 0.02 14 V 0 -3 -2 -3 -1 -2 -3 2 -3 2 0 -2 0 -2 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 26 0 0 0 0 0 0 0 0 0 0 0 74 0.27 0.02 15 N -2 0 6 1 -3 3 0 -1 0 -4 -4 0 -2 -4 -2 0 0 -4 -2 -3 0 0 74 0 0 26 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.63 0.03 16 S -1 -2 -2 -2 -2 -2 -2 -2 -2 2 0 -2 0 5 -3 1 -1 -1 1 0 0 0 0 0 0 0 0 0 0 26 0 0 0 45 0 28 0 0 0 0 0.24 0.02 17 V -2 -3 -4 -4 -2 -3 -3 -4 -2 1 0 -3 0 6 -4 -3 -2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 72 0 0 0 0 0 28 0.53 0.02 18 L -2 -3 -4 -4 -2 -3 -4 -4 -3 3 3 -3 1 4 -3 -3 -1 -1 1 2 0 0 0 0 0 0 0 0 0 45 28 0 0 26 0 0 0 0 0 0 0.34 0.01 19 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 4 -3 2 3 -3 -3 -2 -1 0 1 0 0 0 0 0 0 0 0 0 0 84 0 0 16 0 0 0 0 0 0 0.44 0.02 20 F 0 -2 -1 -2 -1 -2 -2 -2 -2 1 0 -2 0 3 -2 1 3 -1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 28 0 16 30 0 0 26 0.18 0.01 21 L -1 -3 -4 -4 -1 -3 -3 -4 -3 2 3 -3 2 0 -3 -2 -1 -2 -1 3 0 0 0 0 0 0 0 0 0 0 55 0 0 0 0 0 0 0 0 45 0.35 0.01 22 A 2 -2 -1 -2 -1 -1 -1 -1 -2 1 0 -1 0 -2 -2 2 0 -3 -2 3 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 0 0 0 45 0.19 0.01 23 F -1 -4 -3 -4 9 -4 -4 -3 -3 -1 -1 -4 -1 3 -4 -2 -2 -1 0 -1 0 0 0 0 72 0 0 0 0 0 0 0 0 28 0 0 0 0 0 0 0.92 0.03 24 V 3 -2 -3 -3 -1 -2 -2 -1 -3 1 0 -2 0 -2 -2 0 0 -3 -2 3 45 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 55 0.24 0.02 25 V -1 -3 -4 -4 -1 -3 -3 -4 -4 4 1 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 56 0 0 0 0 0 0 0 0 0 44 0.39 0.02 26 F -1 -2 -2 -3 -2 -2 -2 -3 -2 -1 0 -2 0 5 -3 0 3 0 2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 55 0 0 45 0 0 0 0.39 0.02 27 L -2 -3 -4 -4 -2 -3 -3 -4 -3 2 5 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.51 0.02 28 L -1 -3 -4 -4 -1 -3 -3 -4 -3 3 4 -3 2 0 -3 -3 -1 -2 -1 2 0 0 0 0 0 0 0 0 0 26 58 0 0 0 0 0 0 0 0 16 0.37 0.01 29 V -1 -3 -3 -4 -1 -3 -3 -4 -3 2 1 -3 0 3 -3 -2 -1 -2 1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 26 0 0 0 0 0 74 0.33 0.02 30 T 0 -3 -2 -3 8 -2 -3 -3 -3 0 -1 -2 -1 -2 -3 0 2 -3 -2 2 0 0 0 0 45 0 0 0 0 0 0 0 0 0 0 0 28 0 0 26 0.50 0.02 31 L -1 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 3 0 -3 -2 -1 -2 -1 2 0 0 0 0 0 0 0 0 0 0 57 0 16 0 0 0 0 0 0 26 0.35 0.01 32 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.46 0.03 33 I -2 -3 -4 -4 -2 -3 -3 -4 -3 3 2 -3 1 3 -3 -3 -1 -2 0 2 0 0 0 0 0 0 0 0 0 44 26 0 0 16 0 0 0 0 0 13 0.32 0.01 34 L -2 -3 -4 -4 -2 -3 -3 -4 -3 2 5 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.51 0.02 35 T 2 -1 -1 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -3 -1 1 5 -3 -2 0 26 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 74 0 0 0 0.40 0.02 36 A 4 -2 -1 -2 -1 -1 -1 0 -2 -1 -2 -1 -1 -3 -1 1 2 -3 -2 0 74 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 0 0 0 0.36 0.02 37 L -1 -3 -3 -3 -1 -3 -3 -4 -3 4 3 -3 1 0 -3 -2 1 -3 -2 2 0 0 0 0 0 0 0 0 0 55 28 0 0 0 0 0 16 0 0 0 0.29 0.01 38 R -2 6 -1 -2 -4 1 0 -3 -1 -3 -3 3 -2 -3 -2 -1 -1 -3 -2 -3 0 74 0 0 0 0 0 0 0 0 0 26 0 0 0 0 0 0 0 0 0.68 0.02 39 L -2 -3 -4 -4 -2 -3 -3 -4 -3 2 5 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.51 0.02 40 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.41 0.04 41 A 2 -3 -3 -3 -1 -2 -2 -2 -3 3 1 -2 0 -1 -2 -1 0 -3 -2 3 28 0 0 0 0 0 0 0 0 26 0 0 0 0 0 0 0 0 0 45 0.23 0.01 42 Y -1 0 -1 -1 -3 5 1 -3 1 -3 -2 1 -1 0 -2 -1 -1 0 4 -2 0 0 0 0 0 72 0 0 0 0 0 0 0 0 0 0 0 0 28 0 0.53 0.03 43 C -1 -4 -3 -4 9 -3 -4 -3 -4 2 0 -4 -1 -2 -3 -2 -1 -3 -3 0 0 0 0 0 74 0 0 0 0 26 0 0 0 0 0 0 0 0 0 0 0.91 0.03 44 C 1 -3 -3 -4 8 -2 -3 -2 -3 0 0 -3 2 -2 -3 -1 -1 -3 -2 1 13 0 0 0 55 0 0 0 0 0 0 0 16 0 0 0 0 0 0 16 0.58 0.02 45 N 0 -1 3 0 -2 -1 -1 3 -1 -3 -3 -1 -2 -3 -2 2 2 -3 -2 -2 0 0 28 0 0 0 0 26 0 0 0 0 0 0 0 29 16 0 0 0 0.38 0.01 46 I -1 -3 -2 -3 -2 -3 -3 4 -2 2 0 -3 -1 3 -3 -1 -2 -1 0 0 0 0 0 0 0 0 0 45 0 28 0 0 0 26 0 0 0 0 0 0 0.32 0.01 47 V -1 -3 -3 -4 7 -3 -4 -3 -3 1 0 -3 0 2 -3 -2 -1 -2 0 2 0 0 0 0 42 0 0 0 0 0 0 0 0 16 0 0 0 0 0 42 0.47 0.02 48 N -2 -1 6 1 -3 0 0 -1 4 -4 -4 0 -2 -3 -2 0 0 -4 -1 -3 0 0 84 0 0 0 0 0 16 0 0 0 0 0 0 0 0 0 0 0 0.70 0.03 49 V -1 -2 -2 -2 -1 -2 -2 -3 -3 3 0 -2 0 -1 -2 0 3 -3 -2 3 0 0 0 0 0 0 0 0 0 26 0 0 0 0 0 0 45 0 0 28 0.26 0.01 50 S -1 -2 -2 -3 -2 -2 -2 -3 -2 0 3 -2 1 3 -3 1 0 -1 1 0 0 0 0 0 0 0 0 0 0 0 45 0 0 26 0 28 0 0 0 0 0.20 0.01 51 L -1 -3 -4 -4 -1 -3 -3 -4 -3 3 4 -3 2 0 -3 -3 -1 -2 -1 2 0 0 0 0 0 0 0 0 0 26 58 0 0 0 0 0 0 0 0 16 0.37 0.01 52 V -1 -3 -3 -4 -2 -3 -3 -4 -3 3 1 -3 1 4 -3 -2 -1 -1 1 3 0 0 0 0 0 0 0 0 0 26 0 0 0 29 0 0 0 0 0 45 0.32 0.01 53 K 0 0 -1 -1 -2 2 0 -2 -1 0 0 2 0 -2 -2 2 0 -3 -2 0 0 0 0 0 0 16 0 0 0 0 16 28 0 0 0 26 0 0 0 13 0.14 0.01 54 P -1 -3 -2 -2 -3 -2 -1 -3 -3 -3 -3 -1 -3 -4 8 -1 -1 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.11 0.04 55 S 3 -1 0 -1 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 3 2 -3 -2 -1 45 0 0 0 0 0 0 0 0 0 0 0 0 0 0 40 14 0 0 0 0.34 0.01 56 F 1 -3 -3 -3 -1 -2 -3 -2 -2 1 1 -2 0 3 -3 -1 -1 -1 1 2 26 0 0 0 0 0 0 0 0 0 16 0 0 27 0 0 0 0 0 30 0.18 0.01 57 Y -2 -2 -2 -3 -3 -1 -2 -3 4 -2 -2 -2 -1 3 -3 -2 -2 2 7 -2 0 0 0 0 0 0 0 0 16 0 0 0 0 0 0 0 0 0 84 0 0.85 0.03 58 V -1 -3 -4 -4 -1 -3 -3 -4 -4 3 2 -3 1 -1 -3 -2 -1 -3 -1 4 0 0 0 0 0 0 0 0 0 29 16 0 0 0 0 0 0 0 0 55 0.35 0.01 59 Y -2 -2 -3 -4 -3 -2 -3 -4 2 -2 -1 -2 -1 3 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.00 0.04 60 S -1 -1 6 1 -2 0 0 -1 0 -3 -4 0 -2 -3 -2 2 0 -4 -2 -3 0 0 72 0 0 0 0 0 0 0 0 0 0 0 0 28 0 0 0 0 0.59 0.02 61 R -1 5 0 -2 -3 0 -1 -2 -1 -2 -2 1 -1 -3 -2 0 3 -3 -2 -2 0 61 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0.46 0.02 62 V 0 -3 -1 -2 -2 -2 -3 5 -3 0 -2 -2 -1 -3 -3 -1 -1 -3 -3 1 0 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0 0 0 33 0.53 0.01 63 K -1 6 0 -2 -4 1 0 -2 -1 -3 -3 4 -2 -3 -2 -1 -1 -3 -2 -3 0 67 0 0 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0.64 0.02 64 N -1 0 5 1 -3 3 1 -1 0 -3 -3 0 -2 -3 -2 2 0 -3 -2 -3 0 0 52 0 0 28 0 0 0 0 0 0 0 0 0 20 0 0 0 0 0.47 0.02 65 L -2 -3 -4 -4 -2 -3 -3 -4 -2 1 3 -3 1 5 -4 -3 -2 0 1 0 0 0 0 0 0 0 0 0 0 0 61 0 0 39 0 0 0 0 0 0 0.43 0.01 66 N -2 0 4 0 -3 4 0 -2 1 -3 -2 0 -1 0 -2 0 -1 -1 4 -2 0 0 33 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 28 0 0.39 0.02 67 S 0 0 0 3 -3 1 2 -1 -1 -3 -3 2 -2 -3 -1 2 0 -4 -2 -3 0 0 0 20 0 0 19 0 0 0 0 28 0 0 0 33 0 0 0 0 0.36 0.01 68 S 1 -1 3 0 -1 0 0 0 -1 -3 -3 0 -2 -3 -1 4 1 -3 -2 -2 0 0 19 0 0 0 0 0 0 0 0 0 0 0 0 81 0 0 0 0 0.45 0.02 69 R -1 5 0 0 -4 1 2 -2 0 -3 -3 3 -2 -3 -2 -1 -1 -3 -2 -3 0 49 0 0 0 0 23 0 0 0 0 28 0 0 0 0 0 0 0 0 0.52 0.00 70 V -1 -3 -3 -2 -2 -2 -2 -3 -3 1 -1 -2 -1 -2 6 -1 -1 -4 -2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 54 0 0 0 0 46 0.86 0.01 71 P -1 -2 -2 -2 -3 -2 -1 -3 -3 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.06 0.01 72 D -2 -2 1 7 -4 0 2 -2 -1 -4 -4 -1 -3 -4 -2 0 -1 -5 -3 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.92 0.00 73 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.00 74 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.00 75 V 0 -3 -3 -4 -1 -3 -3 -4 -4 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.34 0.00 K Lambda Standard Ungapped 0.1416 0.3315 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1416 0.3315 PSI Gapped 0.0410 0.2670