PSIBLAST 2.11.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Stephen F.
Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005)
"Protein database searches using compositionally adjusted
substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.



Database: uniprot_sprot.fasta
           571,282 sequences; 206,678,396 total letters

Results from round 1


Query= YP_009725255.1 ORF10 protein [Severe acute respiratory syndrome
coronavirus 2]

Length=38
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

sp|A0A663DJA2|ORF10_SARS2 Putative ORF10 protein OS=Severe acute ...  78.6    5e-21
sp|A6TL10|ASSY_ALKMQ Argininosuccinate synthase OS=Alkaliphilus m...  27.7    2.2  


>sp|A0A663DJA2|ORF10_SARS2 Putative ORF10 protein OS=Severe acute 
respiratory syndrome coronavirus 2 OX=2697049 GN=ORF10 PE=5 
SV=1
Length=38

 Score = 78.6 bits (192),  Expect = 5e-21, Method: Compositional matrix adjust.
 Identities = 38/38 (100%), Positives = 38/38 (100%), Gaps = 0/38 (0%)

Query  1   MGYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLT  38
           MGYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLT
Sbjct  1   MGYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLT  38


>sp|A6TL10|ASSY_ALKMQ Argininosuccinate synthase OS=Alkaliphilus 
metalliredigens (strain QYMF) OX=293826 GN=argG PE=3 SV=1
Length=414

 Score = 27.7 bits (60),  Expect = 2.2, Method: Composition-based stats.
 Identities = 10/17 (59%), Positives = 14/17 (82%), Gaps = 0/17 (0%)

Query  2    GYINVFAFPFTIYSLLL  18
            G+IN+FA P TI +L+L
Sbjct  384  GFINLFALPLTIRALML  400



Lambda      K        H        a         alpha
   0.335    0.145    0.443    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 5195398312
Results from round 2


Query= YP_009725255.1 ORF10 protein [Severe acute respiratory syndrome
coronavirus 2]

Length=38
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value
Sequences used in model and found again:

sp|A0A663DJA2|ORF10_SARS2 Putative ORF10 protein OS=Severe acute ...  57.7    8e-13

Sequences not found previously or not previously below threshold:

sp|A6TL10|ASSY_ALKMQ Argininosuccinate synthase OS=Alkaliphilus m...  26.4    6.7  


>sp|A0A663DJA2|ORF10_SARS2 Putative ORF10 protein OS=Severe acute 
respiratory syndrome coronavirus 2 OX=2697049 GN=ORF10 PE=5 
SV=1
Length=38

 Score = 57.7 bits (138),  Expect = 8e-13, Method: Composition-based stats.
 Identities = 38/38 (100%), Positives = 38/38 (100%), Gaps = 0/38 (0%)

Query  1   MGYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLT  38
           MGYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLT
Sbjct  1   MGYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLT  38


>sp|A6TL10|ASSY_ALKMQ Argininosuccinate synthase OS=Alkaliphilus 
metalliredigens (strain QYMF) OX=293826 GN=argG PE=3 SV=1
Length=414

 Score = 26.4 bits (57),  Expect = 6.7, Method: Composition-based stats.
 Identities = 10/17 (59%), Positives = 14/17 (82%), Gaps = 0/17 (0%)

Query  2    GYINVFAFPFTIYSLLL  18
            G+IN+FA P TI +L+L
Sbjct  384  GFINLFALPLTIRALML  400



Lambda      K        H        a         alpha
   0.321    0.155    0.528    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0475    0.140     1.90     42.6     43.6 

Effective search space used: 5195398312

Search has CONVERGED!


  Database: uniprot_sprot.fasta
    Posted date:  Apr 3, 2024  12:05 PM
  Number of letters in database: 206,678,396
  Number of sequences in database:  571,282



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40