PSIBLAST 2.11.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Stephen F.
Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005)
"Protein database searches using compositionally adjusted
substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: uniprot_sprot.fasta
571,282 sequences; 206,678,396 total letters
Results from round 1
Query= YP_009724394.1 ORF6 protein [Severe acute respiratory syndrome
coronavirus 2]
Length=61
Score E
Sequences producing significant alignments: (Bits) Value
sp|P0DTC6|NS6_SARS2 ORF6 protein OS=Severe acute respiratory synd... 122 9e-38
sp|Q3I5J1|NS6_BCRP3 ORF6 protein OS=Bat coronavirus Rp3/2004 OX=3... 92.4 7e-26
sp|Q0Q471|NS6_BC279 ORF6 protein OS=Bat coronavirus 279/2005 OX=3... 92.4 7e-26
sp|P59634|NS6_SARS ORF6 protein OS=Severe acute respiratory syndr... 92.0 9e-26
sp|Q3LZX8|NS6_BCHK3 ORF6 protein OS=Bat coronavirus HKU3 OX=44273... 90.1 6e-25
sp|Q9LH74|MSL5_ARATH Mechanosensitive ion channel protein 5 OS=Ar... 28.5 2.6
sp|Q6MTC8|PLSX_MYCMS Phosphate acyltransferase OS=Mycoplasma myco... 27.7 5.9
sp|Q92828|COR2A_HUMAN Coronin-2A OS=Homo sapiens OX=9606 GN=CORO2... 27.7 6.4
>sp|P0DTC6|NS6_SARS2 ORF6 protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=6 PE=1 SV=1
Length=61
Score = 122 bits (306), Expect = 9e-38, Method: Compositional matrix adjust.
Identities = 61/61 (100%), Positives = 61/61 (100%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI
Sbjct 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|Q3I5J1|NS6_BCRP3 ORF6 protein OS=Bat coronavirus Rp3/2004
OX=349344 GN=6 PE=3 SV=1
Length=63
Score = 92.4 bits (228), Expect = 7e-26, Method: Compositional matrix adjust.
Identities = 42/61 (69%), Positives = 55/61 (90%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
MFHLVDFQVTIAEIL+IIM+TF+V+IWNLD +I+ I++ L K LT+ KYS+LD+E+PME+
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKKYSELDDEEPMEL 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|Q0Q471|NS6_BC279 ORF6 protein OS=Bat coronavirus 279/2005
OX=389167 GN=6 PE=3 SV=1
Length=63
Score = 92.4 bits (228), Expect = 7e-26, Method: Compositional matrix adjust.
Identities = 42/61 (69%), Positives = 55/61 (90%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
MFHLVDFQVTIAEIL+IIM+TF+V+IWNLD +I+ I++ L K LT+ KYS+LD+E+PME+
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKKYSELDDEEPMEL 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|P59634|NS6_SARS ORF6 protein OS=Severe acute respiratory syndrome
coronavirus OX=694009 GN=6 PE=1 SV=1
Length=63
Score = 92.0 bits (227), Expect = 9e-26, Method: Compositional matrix adjust.
Identities = 42/61 (69%), Positives = 54/61 (89%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
MFHLVDFQVTIAEIL+IIMRTF+++IWNLD II+ I++ L K LT+ YS+LD+E+PME+
Sbjct 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|Q3LZX8|NS6_BCHK3 ORF6 protein OS=Bat coronavirus HKU3 OX=442736
GN=6 PE=3 SV=1
Length=63
Score = 90.1 bits (222), Expect = 6e-25, Method: Compositional matrix adjust.
Identities = 41/61 (67%), Positives = 54/61 (89%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
MFHLVDFQVTIAEIL+IIM+TF+V+IWNLD +I+ I++ L K LT+ YS+LD+E+PME+
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKNYSELDDEEPMEL 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|Q9LH74|MSL5_ARATH Mechanosensitive ion channel protein 5 OS=Arabidopsis
thaliana OX=3702 GN=MSL5 PE=2 SV=1
Length=881
Score = 28.5 bits (62), Expect = 2.6, Method: Composition-based stats.
Identities = 12/38 (32%), Positives = 21/38 (55%), Gaps = 0/38 (0%)
Query 19 MRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQ 56
M T VS WN+ ++N+I+K +L +N E++
Sbjct 509 MNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDE 546
>sp|Q6MTC8|PLSX_MYCMS Phosphate acyltransferase OS=Mycoplasma
mycoides subsp. mycoides SC (strain PG1) OX=272632 GN=plsX PE=3
SV=1
Length=334
Score = 27.7 bits (60), Expect = 5.9, Method: Compositional matrix adjust.
Identities = 14/32 (44%), Positives = 21/32 (66%), Gaps = 1/32 (3%)
Query 19 MRTFKVSI-WNLDYIINLIIKNLSKSLTENKY 49
+R+FK ++ LD I N I+K + K L EN+Y
Sbjct 303 VRSFKATLRMTLDAIKNDIVKKIEKGLIENEY 334
>sp|Q92828|COR2A_HUMAN Coronin-2A OS=Homo sapiens OX=9606 GN=CORO2A
PE=1 SV=2
Length=525
Score = 27.7 bits (60), Expect = 6.4, Method: Composition-based stats.
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 0/38 (0%)
Query 4 LVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLS 41
LV++ T A IL +KV IWNLD ++I +S
Sbjct 137 LVEWHPTAANILFSAGYDYKVMIWNLDTKESVITSPMS 174
Lambda K H a alpha
0.325 0.140 0.389 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 5055879816
Results from round 2
Query= YP_009724394.1 ORF6 protein [Severe acute respiratory syndrome
coronavirus 2]
Length=61
Score E
Sequences producing significant alignments: (Bits) Value
Sequences used in model and found again:
sp|P59634|NS6_SARS ORF6 protein OS=Severe acute respiratory syndr... 88.8 2e-24
sp|Q3LZX8|NS6_BCHK3 ORF6 protein OS=Bat coronavirus HKU3 OX=44273... 88.0 4e-24
sp|Q3I5J1|NS6_BCRP3 ORF6 protein OS=Bat coronavirus Rp3/2004 OX=3... 88.0 4e-24
sp|Q0Q471|NS6_BC279 ORF6 protein OS=Bat coronavirus 279/2005 OX=3... 88.0 4e-24
sp|P0DTC6|NS6_SARS2 ORF6 protein OS=Severe acute respiratory synd... 81.1 2e-21
Sequences not found previously or not previously below threshold:
>sp|P59634|NS6_SARS ORF6 protein OS=Severe acute respiratory syndrome
coronavirus OX=694009 GN=6 PE=1 SV=1
Length=63
Score = 88.8 bits (219), Expect = 2e-24, Method: Composition-based stats.
Identities = 42/61 (69%), Positives = 54/61 (89%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
MFHLVDFQVTIAEIL+IIMRTF+++IWNLD II+ I++ L K LT+ YS+LD+E+PME+
Sbjct 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|Q3LZX8|NS6_BCHK3 ORF6 protein OS=Bat coronavirus HKU3 OX=442736
GN=6 PE=3 SV=1
Length=63
Score = 88.0 bits (217), Expect = 4e-24, Method: Composition-based stats.
Identities = 41/61 (67%), Positives = 54/61 (89%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
MFHLVDFQVTIAEIL+IIM+TF+V+IWNLD +I+ I++ L K LT+ YS+LD+E+PME+
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKNYSELDDEEPMEL 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|Q3I5J1|NS6_BCRP3 ORF6 protein OS=Bat coronavirus Rp3/2004
OX=349344 GN=6 PE=3 SV=1
Length=63
Score = 88.0 bits (217), Expect = 4e-24, Method: Composition-based stats.
Identities = 42/61 (69%), Positives = 55/61 (90%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
MFHLVDFQVTIAEIL+IIM+TF+V+IWNLD +I+ I++ L K LT+ KYS+LD+E+PME+
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKKYSELDDEEPMEL 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|Q0Q471|NS6_BC279 ORF6 protein OS=Bat coronavirus 279/2005
OX=389167 GN=6 PE=3 SV=1
Length=63
Score = 88.0 bits (217), Expect = 4e-24, Method: Composition-based stats.
Identities = 42/61 (69%), Positives = 55/61 (90%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
MFHLVDFQVTIAEIL+IIM+TF+V+IWNLD +I+ I++ L K LT+ KYS+LD+E+PME+
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKKYSELDDEEPMEL 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|P0DTC6|NS6_SARS2 ORF6 protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=6 PE=1 SV=1
Length=61
Score = 81.1 bits (199), Expect = 2e-21, Method: Composition-based stats.
Identities = 61/61 (100%), Positives = 61/61 (100%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI
Sbjct 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
Query 61 D 61
D
Sbjct 61 D 61
Lambda K H a alpha
0.322 0.151 0.441 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0461 0.140 1.90 42.6 43.6
Effective search space used: 5055879816
Search has CONVERGED!
Database: uniprot_sprot.fasta
Posted date: Apr 3, 2024 12:05 PM
Number of letters in database: 206,678,396
Number of sequences in database: 571,282
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40