PSIBLAST 2.11.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Stephen F.
Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005)
"Protein database searches using compositionally adjusted
substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: uniprot_sprot.fasta
571,282 sequences; 206,678,396 total letters
Results from round 1
Query= sp|Q7TFA1|NS7B_SARS Protein non-structural 7b OS=Severe acute
respiratory syndrome coronavirus OX=694009 GN=7b PE=1 SV=1
Length=44
Score E
Sequences producing significant alignments: (Bits) Value
sp|Q7TFA1|NS7B_SARS Protein non-structural 7b OS=Severe acute res... 85.1 2e-23
sp|Q3I5I9|NS7B_BCRP3 Non-structural protein 7b OS=Bat coronavirus... 53.9 4e-11
sp|P0C5A9|NS7B_BC279 Non-structural protein 7b OS=Bat coronavirus... 53.9 4e-11
sp|Q3LZX6|NS7B_BCHK3 Non-structural protein 7b OS=Bat coronavirus... 46.6 3e-08
sp|P0DTD8|NS7B_SARS2 ORF7b protein OS=Severe acute respiratory sy... 38.5 5e-05
sp|Q9M883|SC5D2_ARATH Putative Delta(7)-sterol-C5(6)-desaturase 2... 30.8 0.21
>sp|Q7TFA1|NS7B_SARS Protein non-structural 7b OS=Severe acute
respiratory syndrome coronavirus OX=694009 GN=7b PE=1 SV=1
Length=44
Score = 85.1 bits (209), Expect = 2e-23, Method: Compositional matrix adjust.
Identities = 44/44 (100%), Positives = 44/44 (100%), Gaps = 0/44 (0%)
Query 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV 44
MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV 44
>sp|Q3I5I9|NS7B_BCRP3 Non-structural protein 7b OS=Bat coronavirus
Rp3/2004 OX=349344 GN=7b PE=4 SV=1
Length=44
Score = 53.9 bits (128), Expect = 4e-11, Method: Compositional matrix adjust.
Identities = 41/44 (93%), Positives = 43/44 (98%), Gaps = 0/44 (0%)
Query 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV 44
MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLE+QD+EEPC KV
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDIEEPCNKV 44
>sp|P0C5A9|NS7B_BC279 Non-structural protein 7b OS=Bat coronavirus
279/2005 OX=389167 GN=7b PE=4 SV=1
Length=44
Score = 53.9 bits (128), Expect = 4e-11, Method: Compositional matrix adjust.
Identities = 41/44 (93%), Positives = 43/44 (98%), Gaps = 0/44 (0%)
Query 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV 44
MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLE+QD+EEPC KV
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDIEEPCNKV 44
>sp|Q3LZX6|NS7B_BCHK3 Non-structural protein 7b OS=Bat coronavirus
HKU3 OX=442736 GN=7b PE=4 SV=1
Length=44
Score = 46.6 bits (109), Expect = 3e-08, Method: Compositional matrix adjust.
Identities = 41/44 (93%), Positives = 43/44 (98%), Gaps = 0/44 (0%)
Query 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV 44
MNELTLIDFYLCFLAFLLFLVLIML+IFWFSLEIQD+EEPC KV
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLLIFWFSLEIQDIEEPCNKV 44
>sp|P0DTD8|NS7B_SARS2 ORF7b protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=7b PE=1 SV=1
Length=43
Score = 38.5 bits (88), Expect = 5e-05, Method: Compositional matrix adjust.
Identities = 35/41 (85%), Positives = 37/41 (90%), Gaps = 0/41 (0%)
Query 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPC 41
M EL+LIDFYLCFLAFLLFLVLIMLIIFWFSLE+QD E C
Sbjct 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETC 41
>sp|Q9M883|SC5D2_ARATH Putative Delta(7)-sterol-C5(6)-desaturase
2 OS=Arabidopsis thaliana OX=3702 GN=HDF7 PE=3 SV=1
Length=279
Score = 30.8 bits (68), Expect = 0.21, Method: Compositional matrix adjust.
Identities = 12/31 (39%), Positives = 22/31 (71%), Gaps = 0/31 (0%)
Query 8 DFYLCFLAFLLFLVLIMLIIFWFSLEIQDLE 38
+++LCFL L+LVL+ +I+W E+ D++
Sbjct 125 NWFLCFLYIALYLVLVEFMIYWVHKELHDIK 155
Lambda K H a alpha
0.342 0.155 0.498 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 5106278320
Results from round 2
Query= sp|Q7TFA1|NS7B_SARS Protein non-structural 7b OS=Severe acute
respiratory syndrome coronavirus OX=694009 GN=7b PE=1 SV=1
Length=44
Score E
Sequences producing significant alignments: (Bits) Value
Sequences used in model and found again:
sp|Q7TFA1|NS7B_SARS Protein non-structural 7b OS=Severe acute res... 45.9 5e-08
sp|Q3I5I9|NS7B_BCRP3 Non-structural protein 7b OS=Bat coronavirus... 34.0 0.003
sp|P0C5A9|NS7B_BC279 Non-structural protein 7b OS=Bat coronavirus... 34.0 0.003
sp|Q3LZX6|NS7B_BCHK3 Non-structural protein 7b OS=Bat coronavirus... 32.1 0.018
sp|P0DTD8|NS7B_SARS2 ORF7b protein OS=Severe acute respiratory sy... 30.1 0.11
Sequences not found previously or not previously below threshold:
sp|Q9M883|SC5D2_ARATH Putative Delta(7)-sterol-C5(6)-desaturase 2... 26.7 6.8
>sp|Q7TFA1|NS7B_SARS Protein non-structural 7b OS=Severe acute
respiratory syndrome coronavirus OX=694009 GN=7b PE=1 SV=1
Length=44
Score = 45.9 bits (108), Expect = 5e-08, Method: Composition-based stats.
Identities = 44/44 (100%), Positives = 44/44 (100%), Gaps = 0/44 (0%)
Query 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV 44
MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV 44
>sp|Q3I5I9|NS7B_BCRP3 Non-structural protein 7b OS=Bat coronavirus
Rp3/2004 OX=349344 GN=7b PE=4 SV=1
Length=44
Score = 34.0 bits (77), Expect = 0.003, Method: Composition-based stats.
Identities = 41/44 (93%), Positives = 43/44 (98%), Gaps = 0/44 (0%)
Query 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV 44
MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLE+QD+EEPC KV
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDIEEPCNKV 44
>sp|P0C5A9|NS7B_BC279 Non-structural protein 7b OS=Bat coronavirus
279/2005 OX=389167 GN=7b PE=4 SV=1
Length=44
Score = 34.0 bits (77), Expect = 0.003, Method: Composition-based stats.
Identities = 41/44 (93%), Positives = 43/44 (98%), Gaps = 0/44 (0%)
Query 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV 44
MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLE+QD+EEPC KV
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDIEEPCNKV 44
>sp|Q3LZX6|NS7B_BCHK3 Non-structural protein 7b OS=Bat coronavirus
HKU3 OX=442736 GN=7b PE=4 SV=1
Length=44
Score = 32.1 bits (72), Expect = 0.018, Method: Composition-based stats.
Identities = 41/44 (93%), Positives = 43/44 (98%), Gaps = 0/44 (0%)
Query 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTKV 44
MNELTLIDFYLCFLAFLLFLVLIML+IFWFSLEIQD+EEPC KV
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLLIFWFSLEIQDIEEPCNKV 44
>sp|P0DTD8|NS7B_SARS2 ORF7b protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=7b PE=1 SV=1
Length=43
Score = 30.1 bits (67), Expect = 0.11, Method: Composition-based stats.
Identities = 35/41 (85%), Positives = 37/41 (90%), Gaps = 0/41 (0%)
Query 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPC 41
M EL+LIDFYLCFLAFLLFLVLIMLIIFWFSLE+QD E C
Sbjct 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETC 41
>sp|Q9M883|SC5D2_ARATH Putative Delta(7)-sterol-C5(6)-desaturase
2 OS=Arabidopsis thaliana OX=3702 GN=HDF7 PE=3 SV=1
Length=279
Score = 26.7 bits (58), Expect = 6.8, Method: Composition-based stats.
Identities = 13/35 (37%), Positives = 23/35 (66%), Gaps = 0/35 (0%)
Query 4 LTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLE 38
L +++LCFL L+LVL+ +I+W E+ D++
Sbjct 121 LDHFNWFLCFLYIALYLVLVEFMIYWVHKELHDIK 155
Lambda K H a alpha
0.323 0.164 0.577 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0501 0.140 1.90 42.6 43.6
Effective search space used: 5106278320
Search has CONVERGED!
Database: uniprot_sprot.fasta
Posted date: Apr 3, 2024 12:05 PM
Number of letters in database: 206,678,396
Number of sequences in database: 571,282
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40