PSIBLAST 2.11.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Stephen F.
Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005)
"Protein database searches using compositionally adjusted
substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: uniprot_sprot.fasta
571,282 sequences; 206,678,396 total letters
Results from round 1
Query= sp|P59634|NS6_SARS ORF6 protein OS=Severe acute respiratory syndrome
coronavirus OX=694009 GN=6 PE=1 SV=1
Length=63
Score E
Sequences producing significant alignments: (Bits) Value
sp|P59634|NS6_SARS ORF6 protein OS=Severe acute respiratory syndr... 127 6e-40
sp|Q3LZX8|NS6_BCHK3 ORF6 protein OS=Bat coronavirus HKU3 OX=44273... 124 1e-38
sp|Q3I5J1|NS6_BCRP3 ORF6 protein OS=Bat coronavirus Rp3/2004 OX=3... 122 9e-38
sp|Q0Q471|NS6_BC279 ORF6 protein OS=Bat coronavirus 279/2005 OX=3... 122 9e-38
sp|P0DTC6|NS6_SARS2 ORF6 protein OS=Severe acute respiratory synd... 92.0 1e-25
sp|Q89A29|TRPE_BUCBP Anthranilate synthase component 1 OS=Buchner... 28.1 5.1
sp|P43743|DPO3A_HAEIN DNA polymerase III subunit alpha OS=Haemoph... 27.3 9.7
>sp|P59634|NS6_SARS ORF6 protein OS=Severe acute respiratory syndrome
coronavirus OX=694009 GN=6 PE=1 SV=1
Length=63
Score = 127 bits (320), Expect = 6e-40, Method: Compositional matrix adjust.
Identities = 63/63 (100%), Positives = 63/63 (100%), Gaps = 0/63 (0%)
Query 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL
Sbjct 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
Query 61 DYP 63
DYP
Sbjct 61 DYP 63
>sp|Q3LZX8|NS6_BCHK3 ORF6 protein OS=Bat coronavirus HKU3 OX=442736
GN=6 PE=3 SV=1
Length=63
Score = 124 bits (312), Expect = 1e-38, Method: Compositional matrix adjust.
Identities = 59/63 (94%), Positives = 63/63 (100%), Gaps = 0/63 (0%)
Query 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
MFHLVDFQVTIAEILIIIM+TFR+AIWNLD++ISSIVRQLFKPLTKKNYSELDDEEPMEL
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKNYSELDDEEPMEL 60
Query 61 DYP 63
DYP
Sbjct 61 DYP 63
>sp|Q3I5J1|NS6_BCRP3 ORF6 protein OS=Bat coronavirus Rp3/2004
OX=349344 GN=6 PE=3 SV=1
Length=63
Score = 122 bits (306), Expect = 9e-38, Method: Compositional matrix adjust.
Identities = 58/63 (92%), Positives = 62/63 (98%), Gaps = 0/63 (0%)
Query 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
MFHLVDFQVTIAEILIIIM+TFR+AIWNLD++ISSIVRQLFKPLTKK YSELDDEEPMEL
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKKYSELDDEEPMEL 60
Query 61 DYP 63
DYP
Sbjct 61 DYP 63
>sp|Q0Q471|NS6_BC279 ORF6 protein OS=Bat coronavirus 279/2005
OX=389167 GN=6 PE=3 SV=1
Length=63
Score = 122 bits (306), Expect = 9e-38, Method: Compositional matrix adjust.
Identities = 58/63 (92%), Positives = 62/63 (98%), Gaps = 0/63 (0%)
Query 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
MFHLVDFQVTIAEILIIIM+TFR+AIWNLD++ISSIVRQLFKPLTKK YSELDDEEPMEL
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKKYSELDDEEPMEL 60
Query 61 DYP 63
DYP
Sbjct 61 DYP 63
>sp|P0DTC6|NS6_SARS2 ORF6 protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=6 PE=1 SV=1
Length=61
Score = 92.0 bits (227), Expect = 1e-25, Method: Compositional matrix adjust.
Identities = 42/61 (69%), Positives = 54/61 (89%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
MFHLVDFQVTIAEIL+IIMRTF+++IWNLD II+ I++ L K LT+ YS+LD+E+PME+
Sbjct 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|Q89A29|TRPE_BUCBP Anthranilate synthase component 1 OS=Buchnera
aphidicola subsp. Baizongia pistaciae (strain Bp) OX=224915
GN=trpE PE=3 SV=1
Length=522
Score = 28.1 bits (61), Expect = 5.1, Method: Compositional matrix adjust.
Identities = 17/57 (30%), Positives = 29/57 (51%), Gaps = 6/57 (11%)
Query 13 EILIIIMRTFRIAIWNLDVIISSIVRQ------LFKPLTKKNYSELDDEEPMELDYP 63
E ++II +I+ N VI+ ++ + +FK L KN L D P+E+ +P
Sbjct 50 ESMMIIDAALKISFLNQIVIVEALTKNGVNLLSVFKSLLPKNVIILSDNNPLEIKFP 106
>sp|P43743|DPO3A_HAEIN DNA polymerase III subunit alpha OS=Haemophilus
influenzae (strain ATCC 51907 / DSM 11121 / KW20 /
Rd) OX=71421 GN=dnaE PE=3 SV=1
Length=1159
Score = 27.3 bits (59), Expect = 9.7, Method: Composition-based stats.
Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Query 19 MRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
+RT I W LD+I +VR+ KP LDD E EL
Sbjct 559 LRTLTIIKWALDIINVRMVRE-GKPRVDIAAIPLDDPESFEL 599
Lambda K H a alpha
0.329 0.144 0.420 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 5227138728
Results from round 2
Query= sp|P59634|NS6_SARS ORF6 protein OS=Severe acute respiratory syndrome
coronavirus OX=694009 GN=6 PE=1 SV=1
Length=63
Score E
Sequences producing significant alignments: (Bits) Value
Sequences used in model and found again:
sp|P59634|NS6_SARS ORF6 protein OS=Severe acute respiratory syndr... 95.7 3e-27
sp|Q3LZX8|NS6_BCHK3 ORF6 protein OS=Bat coronavirus HKU3 OX=44273... 95.4 6e-27
sp|Q3I5J1|NS6_BCRP3 ORF6 protein OS=Bat coronavirus Rp3/2004 OX=3... 94.6 1e-26
sp|Q0Q471|NS6_BC279 ORF6 protein OS=Bat coronavirus 279/2005 OX=3... 94.6 1e-26
sp|P0DTC6|NS6_SARS2 ORF6 protein OS=Severe acute respiratory synd... 84.2 1e-22
Sequences not found previously or not previously below threshold:
sp|Q89A29|TRPE_BUCBP Anthranilate synthase component 1 OS=Buchner... 29.1 1.9
>sp|P59634|NS6_SARS ORF6 protein OS=Severe acute respiratory syndrome
coronavirus OX=694009 GN=6 PE=1 SV=1
Length=63
Score = 95.7 bits (237), Expect = 3e-27, Method: Composition-based stats.
Identities = 63/63 (100%), Positives = 63/63 (100%), Gaps = 0/63 (0%)
Query 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL
Sbjct 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
Query 61 DYP 63
DYP
Sbjct 61 DYP 63
>sp|Q3LZX8|NS6_BCHK3 ORF6 protein OS=Bat coronavirus HKU3 OX=442736
GN=6 PE=3 SV=1
Length=63
Score = 95.4 bits (236), Expect = 6e-27, Method: Composition-based stats.
Identities = 59/63 (94%), Positives = 63/63 (100%), Gaps = 0/63 (0%)
Query 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
MFHLVDFQVTIAEILIIIM+TFR+AIWNLD++ISSIVRQLFKPLTKKNYSELDDEEPMEL
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKNYSELDDEEPMEL 60
Query 61 DYP 63
DYP
Sbjct 61 DYP 63
>sp|Q3I5J1|NS6_BCRP3 ORF6 protein OS=Bat coronavirus Rp3/2004
OX=349344 GN=6 PE=3 SV=1
Length=63
Score = 94.6 bits (234), Expect = 1e-26, Method: Composition-based stats.
Identities = 58/63 (92%), Positives = 62/63 (98%), Gaps = 0/63 (0%)
Query 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
MFHLVDFQVTIAEILIIIM+TFR+AIWNLD++ISSIVRQLFKPLTKK YSELDDEEPMEL
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKKYSELDDEEPMEL 60
Query 61 DYP 63
DYP
Sbjct 61 DYP 63
>sp|Q0Q471|NS6_BC279 ORF6 protein OS=Bat coronavirus 279/2005
OX=389167 GN=6 PE=3 SV=1
Length=63
Score = 94.6 bits (234), Expect = 1e-26, Method: Composition-based stats.
Identities = 58/63 (92%), Positives = 62/63 (98%), Gaps = 0/63 (0%)
Query 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
MFHLVDFQVTIAEILIIIM+TFR+AIWNLD++ISSIVRQLFKPLTKK YSELDDEEPMEL
Sbjct 1 MFHLVDFQVTIAEILIIIMKTFRVAIWNLDILISSIVRQLFKPLTKKKYSELDDEEPMEL 60
Query 61 DYP 63
DYP
Sbjct 61 DYP 63
>sp|P0DTC6|NS6_SARS2 ORF6 protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=6 PE=1 SV=1
Length=61
Score = 84.2 bits (207), Expect = 1e-22, Method: Composition-based stats.
Identities = 42/61 (69%), Positives = 54/61 (89%), Gaps = 0/61 (0%)
Query 1 MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLTKKNYSELDDEEPMEL 60
MFHLVDFQVTIAEIL+IIMRTF+++IWNLD II+ I++ L K LT+ YS+LD+E+PME+
Sbjct 1 MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEI 60
Query 61 D 61
D
Sbjct 61 D 61
>sp|Q89A29|TRPE_BUCBP Anthranilate synthase component 1 OS=Buchnera
aphidicola subsp. Baizongia pistaciae (strain Bp) OX=224915
GN=trpE PE=3 SV=1
Length=522
Score = 29.1 bits (64), Expect = 1.9, Method: Composition-based stats.
Identities = 17/57 (30%), Positives = 29/57 (51%), Gaps = 6/57 (11%)
Query 13 EILIIIMRTFRIAIWNLDVIISSIVRQ------LFKPLTKKNYSELDDEEPMELDYP 63
E ++II +I+ N VI+ ++ + +FK L KN L D P+E+ +P
Sbjct 50 ESMMIIDAALKISFLNQIVIVEALTKNGVNLLSVFKSLLPKNVIILSDNNPLEIKFP 106
Lambda K H a alpha
0.320 0.150 0.449 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0459 0.140 1.90 42.6 43.6
Effective search space used: 5227138728
Search has CONVERGED!
Database: uniprot_sprot.fasta
Posted date: Apr 3, 2024 12:05 PM
Number of letters in database: 206,678,396
Number of sequences in database: 571,282
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40