PSIBLAST 2.11.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Stephen F.
Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005)
"Protein database searches using compositionally adjusted
substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: uniprot_sprot.fasta
571,282 sequences; 206,678,396 total letters
Results from round 1
Query= sp|P59633|NS3B_SARS ORF3b protein OS=Severe acute respiratory
syndrome coronavirus OX=694009 GN=3b PE=1 SV=1
Length=154
Score E
Sequences producing significant alignments: (Bits) Value
sp|P59633|NS3B_SARS ORF3b protein OS=Severe acute respiratory syn... 301 6e-106
sp|Q13459|MYO9B_HUMAN Unconventional myosin-IXb OS=Homo sapiens O... 32.3 1.7
sp|Q63358|MYO9B_RAT Unconventional myosin-IXb OS=Rattus norvegicu... 30.8 6.0
>sp|P59633|NS3B_SARS ORF3b protein OS=Severe acute respiratory
syndrome coronavirus OX=694009 GN=3b PE=1 SV=1
Length=154
Score = 301 bits (772), Expect = 6e-106, Method: Compositional matrix adjust.
Identities = 154/154 (100%), Positives = 154/154 (100%), Gaps = 0/154 (0%)
Query 1 MMPTTLFAGTHITMTTVYHITVSQIQLSLLKVTAFQHQNSKKTTKLVVILRIGTQVLKTM 60
MMPTTLFAGTHITMTTVYHITVSQIQLSLLKVTAFQHQNSKKTTKLVVILRIGTQVLKTM
Sbjct 1 MMPTTLFAGTHITMTTVYHITVSQIQLSLLKVTAFQHQNSKKTTKLVVILRIGTQVLKTM 60
Query 61 SLYMAISPKFTTSLSLHKLLQTLVLKMLHSSSLTSLLKTHRMCKYTQSTALQELLIQQWI 120
SLYMAISPKFTTSLSLHKLLQTLVLKMLHSSSLTSLLKTHRMCKYTQSTALQELLIQQWI
Sbjct 61 SLYMAISPKFTTSLSLHKLLQTLVLKMLHSSSLTSLLKTHRMCKYTQSTALQELLIQQWI 120
Query 121 QFMMSRRRLLACLCKHKKVSTNLCTHSFRKKQVR 154
QFMMSRRRLLACLCKHKKVSTNLCTHSFRKKQVR
Sbjct 121 QFMMSRRRLLACLCKHKKVSTNLCTHSFRKKQVR 154
>sp|Q13459|MYO9B_HUMAN Unconventional myosin-IXb OS=Homo sapiens
OX=9606 GN=MYO9B PE=1 SV=3
Length=2157
Score = 32.3 bits (72), Expect = 1.7, Method: Composition-based stats.
Identities = 24/85 (28%), Positives = 38/85 (45%), Gaps = 12/85 (14%)
Query 78 KLLQTLVLKMLHSSSLTSLLKTHRMCKYTQSTALQE----------LLIQQWIQFMMSRR 127
+++ TL+ KM + KT K T+ ALQE LL+Q W + ++ RR
Sbjct 915 EVISTLLEKMKIDKRNYQIGKTKVFLKETERQALQETLHREVVRKILLLQSWFRMVLERR 974
Query 128 RLLACLCKHKKVSTNLCTHSFRKKQ 152
L K V+ C S+R ++
Sbjct 975 HFLQ--MKRAAVTIQACWRSYRVRR 997
>sp|Q63358|MYO9B_RAT Unconventional myosin-IXb OS=Rattus norvegicus
OX=10116 GN=Myo9b PE=1 SV=1
Length=1980
Score = 30.8 bits (68), Expect = 6.0, Method: Composition-based stats.
Identities = 19/65 (29%), Positives = 30/65 (46%), Gaps = 12/65 (18%)
Query 98 KTHRMCKYTQSTALQE----------LLIQQWIQFMMSRRRLLACLCKHKKVSTNLCTHS 147
KT K T+ ALQE LL+Q W + ++ RR + KH ++ C S
Sbjct 936 KTKVFLKETERQALQERLHGEVLRRILLLQSWFRMVLERRHFVQ--MKHAALTIQACWRS 993
Query 148 FRKKQ 152
+R ++
Sbjct 994 YRVRR 998
Lambda K H a alpha
0.327 0.130 0.367 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 6669077240
Results from round 2
Query= sp|P59633|NS3B_SARS ORF3b protein OS=Severe acute respiratory
syndrome coronavirus OX=694009 GN=3b PE=1 SV=1
Length=154
Score E
Sequences producing significant alignments: (Bits) Value
Sequences used in model and found again:
sp|P59633|NS3B_SARS ORF3b protein OS=Severe acute respiratory syn... 204 2e-67
Sequences not found previously or not previously below threshold:
sp|Q13459|MYO9B_HUMAN Unconventional myosin-IXb OS=Homo sapiens O... 32.8 1.2
sp|Q63358|MYO9B_RAT Unconventional myosin-IXb OS=Rattus norvegicu... 31.6 3.6
>sp|P59633|NS3B_SARS ORF3b protein OS=Severe acute respiratory
syndrome coronavirus OX=694009 GN=3b PE=1 SV=1
Length=154
Score = 204 bits (519), Expect = 2e-67, Method: Composition-based stats.
Identities = 154/154 (100%), Positives = 154/154 (100%), Gaps = 0/154 (0%)
Query 1 MMPTTLFAGTHITMTTVYHITVSQIQLSLLKVTAFQHQNSKKTTKLVVILRIGTQVLKTM 60
MMPTTLFAGTHITMTTVYHITVSQIQLSLLKVTAFQHQNSKKTTKLVVILRIGTQVLKTM
Sbjct 1 MMPTTLFAGTHITMTTVYHITVSQIQLSLLKVTAFQHQNSKKTTKLVVILRIGTQVLKTM 60
Query 61 SLYMAISPKFTTSLSLHKLLQTLVLKMLHSSSLTSLLKTHRMCKYTQSTALQELLIQQWI 120
SLYMAISPKFTTSLSLHKLLQTLVLKMLHSSSLTSLLKTHRMCKYTQSTALQELLIQQWI
Sbjct 61 SLYMAISPKFTTSLSLHKLLQTLVLKMLHSSSLTSLLKTHRMCKYTQSTALQELLIQQWI 120
Query 121 QFMMSRRRLLACLCKHKKVSTNLCTHSFRKKQVR 154
QFMMSRRRLLACLCKHKKVSTNLCTHSFRKKQVR
Sbjct 121 QFMMSRRRLLACLCKHKKVSTNLCTHSFRKKQVR 154
>sp|Q13459|MYO9B_HUMAN Unconventional myosin-IXb OS=Homo sapiens
OX=9606 GN=MYO9B PE=1 SV=3
Length=2157
Score = 32.8 bits (73), Expect = 1.2, Method: Composition-based stats.
Identities = 24/85 (28%), Positives = 38/85 (45%), Gaps = 12/85 (14%)
Query 78 KLLQTLVLKMLHSSSLTSLLKTHRMCKYTQSTALQE----------LLIQQWIQFMMSRR 127
+++ TL+ KM + KT K T+ ALQE LL+Q W + ++ RR
Sbjct 915 EVISTLLEKMKIDKRNYQIGKTKVFLKETERQALQETLHREVVRKILLLQSWFRMVLERR 974
Query 128 RLLACLCKHKKVSTNLCTHSFRKKQ 152
L K V+ C S+R ++
Sbjct 975 HFLQ--MKRAAVTIQACWRSYRVRR 997
>sp|Q63358|MYO9B_RAT Unconventional myosin-IXb OS=Rattus norvegicus
OX=10116 GN=Myo9b PE=1 SV=1
Length=1980
Score = 31.6 bits (70), Expect = 3.6, Method: Composition-based stats.
Identities = 19/65 (29%), Positives = 30/65 (46%), Gaps = 12/65 (18%)
Query 98 KTHRMCKYTQSTALQE----------LLIQQWIQFMMSRRRLLACLCKHKKVSTNLCTHS 147
KT K T+ ALQE LL+Q W + ++ RR + KH ++ C S
Sbjct 936 KTKVFLKETERQALQERLHGEVLRRILLLQSWFRMVLERRHFVQ--MKHAALTIQACWRS 993
Query 148 FRKKQ 152
+R ++
Sbjct 994 YRVRR 998
Lambda K H a alpha
0.320 0.138 0.420 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0423 0.140 1.90 42.6 43.6
Effective search space used: 6669077240
Search has CONVERGED!
Database: uniprot_sprot.fasta
Posted date: Apr 3, 2024 12:05 PM
Number of letters in database: 206,678,396
Number of sequences in database: 571,282
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40