PSIBLAST 2.11.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Stephen F.
Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005)
"Protein database searches using compositionally adjusted
substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: uniprot_sprot.fasta
571,282 sequences; 206,678,396 total letters
Results from round 1
Query= sp|P59632|AP3A_SARS ORF3a protein OS=Severe acute respiratory
syndrome coronavirus OX=694009 GN=3a PE=1 SV=1
Length=274
Score E
Sequences producing significant alignments: (Bits) Value
sp|P59632|AP3A_SARS ORF3a protein OS=Severe acute respiratory syn... 570 0.0
sp|Q0Q474|AP3A_BC279 Protein 3 OS=Bat coronavirus 279/2005 OX=389... 495 1e-178
sp|Q3I5J4|AP3A_BCRP3 Protein 3 OS=Bat coronavirus Rp3/2004 OX=349... 493 1e-177
sp|Q3LZX0|AP3A_BCHK3 Protein 3 OS=Bat coronavirus HKU3 OX=442736 ... 486 4e-175
sp|P0DTC3|AP3A_SARS2 ORF3a protein OS=Severe acute respiratory sy... 426 4e-151
sp|P03127|VE6_CRPVK Protein E6 OS=Cottontail rabbit papillomaviru... 33.5 1.7
sp|Q08853|ATC_PLAFK Calcium-transporting ATPase OS=Plasmodium fal... 32.7 4.8
sp|O02465|OPS2_MANSE Opsin-2 OS=Manduca sexta OX=7130 GN=OP2 PE=2... 31.2 9.6
>sp|P59632|AP3A_SARS ORF3a protein OS=Severe acute respiratory
syndrome coronavirus OX=694009 GN=3a PE=1 SV=1
Length=274
Score = 570 bits (1470), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 274/274 (100%), Positives = 274/274 (100%), Gaps = 0/274 (0%)
Query 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT
Sbjct 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
Query 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA
Sbjct 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
Query 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL
Sbjct 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
Query 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP
Sbjct 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
Query 241 PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL
Sbjct 241 PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
>sp|Q0Q474|AP3A_BC279 Protein 3 OS=Bat coronavirus 279/2005 OX=389167
GN=3 PE=3 SV=1
Length=274
Score = 495 bits (1275), Expect = 1e-178, Method: Compositional matrix adjust.
Identities = 230/274 (84%), Positives = 255/274 (93%), Gaps = 0/274 (0%)
Query 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
MDLFM FTLG+IT QP K++NASPASTVHATATIPLQASLPFGWLV+GVA LAVFQSA+
Sbjct 1 MDLFMSIFTLGAITRQPAKVENASPASTVHATATIPLQASLPFGWLVVGVALLAVFQSAS 60
Query 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
K+IAL+KRWQLALYKG QF+CNLLLLFVTIYSHLLL+AAGMEAQFLY+YALIY LQ ++
Sbjct 61 KVIALHKRWQLALYKGIQFVCNLLLLFVTIYSHLLLLAAGMEAQFLYIYALIYILQIVSF 120
Query 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
CR IMRCWLCWKC+SKNPLLYDANYFVCWHT+ +DYCIPYNS+TDTIV+T GDG + PKL
Sbjct 121 CRFIMRCWLCWKCRSKNPLLYDANYFVCWHTNCFDYCIPYNSITDTIVLTSGDGTTQPKL 180
Query 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
KEDYQIGGYSED HSGVKDYVV+HGYFTE+YYQLESTQ++TDTG ENATFFI++KLVKD
Sbjct 181 KEDYQIGGYSEDWHSGVKDYVVIHGYFTEIYYQLESTQLSTDTGAENATFFIYSKLVKDV 240
Query 241 PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
+VQIHTIDGSSGV NPAMDPIYDEPTTTTSVPL
Sbjct 241 DHVQIHTIDGSSGVVNPAMDPIYDEPTTTTSVPL 274
>sp|Q3I5J4|AP3A_BCRP3 Protein 3 OS=Bat coronavirus Rp3/2004 OX=349344
GN=3 PE=3 SV=1
Length=274
Score = 493 bits (1268), Expect = 1e-177, Method: Compositional matrix adjust.
Identities = 230/274 (84%), Positives = 254/274 (93%), Gaps = 0/274 (0%)
Query 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
MDLFM FTLG+IT QP KI+NASPASTVHATATIPLQASLPFGWLV+GVA LAVFQSA+
Sbjct 1 MDLFMSIFTLGAITRQPAKIENASPASTVHATATIPLQASLPFGWLVVGVALLAVFQSAS 60
Query 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
K+IAL+KRWQLAL+KG Q +CNLLLLFVTIYSHLLL+AAGMEAQFLY+YALIY LQ ++
Sbjct 61 KVIALHKRWQLALHKGIQLVCNLLLLFVTIYSHLLLLAAGMEAQFLYIYALIYILQIVSF 120
Query 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
CR IMRCWLCWKC+SKNPLLYDANYFVCWHT+ +DYCIPYNS+TDTIV+T GDG + PKL
Sbjct 121 CRFIMRCWLCWKCRSKNPLLYDANYFVCWHTNCFDYCIPYNSITDTIVLTSGDGTTQPKL 180
Query 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
KEDYQIGGYSED HSGVKDYVV+HGYFTEVYYQLESTQ++TDTG ENATFFI++KLVKD
Sbjct 181 KEDYQIGGYSEDWHSGVKDYVVIHGYFTEVYYQLESTQLSTDTGAENATFFIYSKLVKDV 240
Query 241 PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
+VQIHTIDGSSGV NPAMDPIYDEPTTTTSVPL
Sbjct 241 DHVQIHTIDGSSGVVNPAMDPIYDEPTTTTSVPL 274
>sp|Q3LZX0|AP3A_BCHK3 Protein 3 OS=Bat coronavirus HKU3 OX=442736
GN=3 PE=3 SV=1
Length=274
Score = 486 bits (1252), Expect = 4e-175, Method: Compositional matrix adjust.
Identities = 225/274 (82%), Positives = 252/274 (92%), Gaps = 0/274 (0%)
Query 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
MDLFM FTLG+IT P KI+NASPASTVHATATIPLQA+ PFGWL++GVA LAVFQSA+
Sbjct 1 MDLFMSIFTLGAITRNPAKIENASPASTVHATATIPLQATFPFGWLIVGVALLAVFQSAS 60
Query 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
K+IAL++RWQLALYKG Q +CN+LLLFVTIYSHLLL+AA MEAQFLY+YALIY LQ ++
Sbjct 61 KVIALHRRWQLALYKGVQLVCNMLLLFVTIYSHLLLLAACMEAQFLYIYALIYILQIVSF 120
Query 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
CR IMRCWLCWKC+SKNPLLYDANYFVCWHT+NYDYCIPYNSVTDT+V+T GDG + PKL
Sbjct 121 CRFIMRCWLCWKCRSKNPLLYDANYFVCWHTNNYDYCIPYNSVTDTVVITSGDGTNQPKL 180
Query 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
KEDYQIGGYSED HSGVKDYVV++GYFTEVYYQLESTQ++TDTG ENATFFI++KLVKD
Sbjct 181 KEDYQIGGYSEDWHSGVKDYVVIYGYFTEVYYQLESTQLSTDTGAENATFFIYSKLVKDV 240
Query 241 PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
+VQIHTIDGSSGV NPAMDPIYDEPTTTTSVPL
Sbjct 241 DHVQIHTIDGSSGVVNPAMDPIYDEPTTTTSVPL 274
>sp|P0DTC3|AP3A_SARS2 ORF3a protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=3a PE=1 SV=1
Length=275
Score = 426 bits (1095), Expect = 4e-151, Method: Compositional matrix adjust.
Identities = 200/275 (73%), Positives = 235/275 (85%), Gaps = 1/275 (0%)
Query 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
MDLFMR FT+G++T + +I +A+P+ V ATATIP+QASLPFGWL++GVA LAVFQSA+
Sbjct 1 MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSAS 60
Query 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
KII L KRWQLAL KG F+CNLLLLFVT+YSHLLLVAAG+EA FLYLYAL+YFLQ IN
Sbjct 61 KIITLKKRWQLALSKGVHFVCNLLLLFVTVYSHLLLVAAGLEAPFLYLYALVYFLQSINF 120
Query 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
RIIMR WLCWKC+SKNPLLYDANYF+CWHT+ YDYCIPYNSVT +IV+T GDG ++P
Sbjct 121 VRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPIS 180
Query 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
+ DYQIGGY+E SGVKD VV+H YFT YYQL STQ++TDTG+E+ TFFI+NK+V +P
Sbjct 181 EHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEP 240
Query 241 -PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
+VQIHTIDGSSGV NP M+PIYDEPTTTTSVPL
Sbjct 241 EEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL 275
>sp|P03127|VE6_CRPVK Protein E6 OS=Cottontail rabbit papillomavirus
(strain Kansas) OX=31553 GN=E6 PE=3 SV=1
Length=273
Score = 33.5 bits (75), Expect = 1.7, Method: Compositional matrix adjust.
Identities = 22/78 (28%), Positives = 33/78 (42%), Gaps = 1/78 (1%)
Query 41 LPFGWLVIGVAFLAVFQSATKIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAG 100
LPFG + G A + K L W G C +L + +Y HL L A
Sbjct 23 LPFGCIFCGKLLGAAEKQLFKCTGLCIVWHKGWPYGTCRDCTVLSCALDLYCHLALTAPA 82
Query 101 MEAQFLYLYAL-IYFLQC 117
+EA+ L + +F++C
Sbjct 83 LEAEALVGQEISSWFMRC 100
>sp|Q08853|ATC_PLAFK Calcium-transporting ATPase OS=Plasmodium
falciparum (isolate K1 / Thailand) OX=5839 GN=ATP6 PE=3 SV=1
Length=1228
Score = 32.7 bits (73), Expect = 4.8, Method: Compositional matrix adjust.
Identities = 23/56 (41%), Positives = 30/56 (54%), Gaps = 4/56 (7%)
Query 209 EVYYQLESTQITTDTGIENATFFIFNKLVKDPPNVQIHTIDGSSGVANPAMDPIYD 264
+ YY ES+ +T D ++F FNKL KD NV+ T DG G + A DP D
Sbjct 392 DTYYFYESSNLTNDIYAGESSF--FNKL-KDEGNVEALTDDGEEGSIDEA-DPYSD 443
>sp|O02465|OPS2_MANSE Opsin-2 OS=Manduca sexta OX=7130 GN=OP2
PE=2 SV=2
Length=377
Score = 31.2 bits (69), Expect = 9.6, Method: Compositional matrix adjust.
Identities = 20/77 (26%), Positives = 38/77 (49%), Gaps = 0/77 (0%)
Query 7 FFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSATKIIALN 66
F +G+ + + NA A H+T T PL L G +++ VAF+ ++ + ++ L
Sbjct 128 FALMGAYSGIGAGMTNACIAYDRHSTITRPLDGRLSEGKVLLMVAFVWIYSTPWALLPLL 187
Query 67 KRWQLALYKGFQFICNL 83
K W + +G+ C+
Sbjct 188 KIWGRYVPEGYLTSCSF 204
Lambda K H a alpha
0.325 0.139 0.441 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 22184730072
Results from round 2
Query= sp|P59632|AP3A_SARS ORF3a protein OS=Severe acute respiratory
syndrome coronavirus OX=694009 GN=3a PE=1 SV=1
Length=274
Score E
Sequences producing significant alignments: (Bits) Value
Sequences used in model and found again:
sp|Q0Q474|AP3A_BC279 Protein 3 OS=Bat coronavirus 279/2005 OX=389... 501 1e-180
sp|Q3I5J4|AP3A_BCRP3 Protein 3 OS=Bat coronavirus Rp3/2004 OX=349... 499 3e-180
sp|Q3LZX0|AP3A_BCHK3 Protein 3 OS=Bat coronavirus HKU3 OX=442736 ... 496 8e-179
sp|P59632|AP3A_SARS ORF3a protein OS=Severe acute respiratory syn... 492 2e-177
sp|P0DTC3|AP3A_SARS2 ORF3a protein OS=Severe acute respiratory sy... 467 2e-167
Sequences not found previously or not previously below threshold:
>sp|Q0Q474|AP3A_BC279 Protein 3 OS=Bat coronavirus 279/2005 OX=389167
GN=3 PE=3 SV=1
Length=274
Score = 501 bits (1289), Expect = 1e-180, Method: Composition-based stats.
Identities = 230/274 (84%), Positives = 255/274 (93%), Gaps = 0/274 (0%)
Query 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
MDLFM FTLG+IT QP K++NASPASTVHATATIPLQASLPFGWLV+GVA LAVFQSA+
Sbjct 1 MDLFMSIFTLGAITRQPAKVENASPASTVHATATIPLQASLPFGWLVVGVALLAVFQSAS 60
Query 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
K+IAL+KRWQLALYKG QF+CNLLLLFVTIYSHLLL+AAGMEAQFLY+YALIY LQ ++
Sbjct 61 KVIALHKRWQLALYKGIQFVCNLLLLFVTIYSHLLLLAAGMEAQFLYIYALIYILQIVSF 120
Query 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
CR IMRCWLCWKC+SKNPLLYDANYFVCWHT+ +DYCIPYNS+TDTIV+T GDG + PKL
Sbjct 121 CRFIMRCWLCWKCRSKNPLLYDANYFVCWHTNCFDYCIPYNSITDTIVLTSGDGTTQPKL 180
Query 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
KEDYQIGGYSED HSGVKDYVV+HGYFTE+YYQLESTQ++TDTG ENATFFI++KLVKD
Sbjct 181 KEDYQIGGYSEDWHSGVKDYVVIHGYFTEIYYQLESTQLSTDTGAENATFFIYSKLVKDV 240
Query 241 PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
+VQIHTIDGSSGV NPAMDPIYDEPTTTTSVPL
Sbjct 241 DHVQIHTIDGSSGVVNPAMDPIYDEPTTTTSVPL 274
>sp|Q3I5J4|AP3A_BCRP3 Protein 3 OS=Bat coronavirus Rp3/2004 OX=349344
GN=3 PE=3 SV=1
Length=274
Score = 499 bits (1286), Expect = 3e-180, Method: Composition-based stats.
Identities = 230/274 (84%), Positives = 254/274 (93%), Gaps = 0/274 (0%)
Query 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
MDLFM FTLG+IT QP KI+NASPASTVHATATIPLQASLPFGWLV+GVA LAVFQSA+
Sbjct 1 MDLFMSIFTLGAITRQPAKIENASPASTVHATATIPLQASLPFGWLVVGVALLAVFQSAS 60
Query 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
K+IAL+KRWQLAL+KG Q +CNLLLLFVTIYSHLLL+AAGMEAQFLY+YALIY LQ ++
Sbjct 61 KVIALHKRWQLALHKGIQLVCNLLLLFVTIYSHLLLLAAGMEAQFLYIYALIYILQIVSF 120
Query 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
CR IMRCWLCWKC+SKNPLLYDANYFVCWHT+ +DYCIPYNS+TDTIV+T GDG + PKL
Sbjct 121 CRFIMRCWLCWKCRSKNPLLYDANYFVCWHTNCFDYCIPYNSITDTIVLTSGDGTTQPKL 180
Query 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
KEDYQIGGYSED HSGVKDYVV+HGYFTEVYYQLESTQ++TDTG ENATFFI++KLVKD
Sbjct 181 KEDYQIGGYSEDWHSGVKDYVVIHGYFTEVYYQLESTQLSTDTGAENATFFIYSKLVKDV 240
Query 241 PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
+VQIHTIDGSSGV NPAMDPIYDEPTTTTSVPL
Sbjct 241 DHVQIHTIDGSSGVVNPAMDPIYDEPTTTTSVPL 274
>sp|Q3LZX0|AP3A_BCHK3 Protein 3 OS=Bat coronavirus HKU3 OX=442736
GN=3 PE=3 SV=1
Length=274
Score = 496 bits (1276), Expect = 8e-179, Method: Composition-based stats.
Identities = 225/274 (82%), Positives = 252/274 (92%), Gaps = 0/274 (0%)
Query 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
MDLFM FTLG+IT P KI+NASPASTVHATATIPLQA+ PFGWL++GVA LAVFQSA+
Sbjct 1 MDLFMSIFTLGAITRNPAKIENASPASTVHATATIPLQATFPFGWLIVGVALLAVFQSAS 60
Query 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
K+IAL++RWQLALYKG Q +CN+LLLFVTIYSHLLL+AA MEAQFLY+YALIY LQ ++
Sbjct 61 KVIALHRRWQLALYKGVQLVCNMLLLFVTIYSHLLLLAACMEAQFLYIYALIYILQIVSF 120
Query 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
CR IMRCWLCWKC+SKNPLLYDANYFVCWHT+NYDYCIPYNSVTDT+V+T GDG + PKL
Sbjct 121 CRFIMRCWLCWKCRSKNPLLYDANYFVCWHTNNYDYCIPYNSVTDTVVITSGDGTNQPKL 180
Query 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
KEDYQIGGYSED HSGVKDYVV++GYFTEVYYQLESTQ++TDTG ENATFFI++KLVKD
Sbjct 181 KEDYQIGGYSEDWHSGVKDYVVIYGYFTEVYYQLESTQLSTDTGAENATFFIYSKLVKDV 240
Query 241 PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
+VQIHTIDGSSGV NPAMDPIYDEPTTTTSVPL
Sbjct 241 DHVQIHTIDGSSGVVNPAMDPIYDEPTTTTSVPL 274
>sp|P59632|AP3A_SARS ORF3a protein OS=Severe acute respiratory
syndrome coronavirus OX=694009 GN=3a PE=1 SV=1
Length=274
Score = 492 bits (1267), Expect = 2e-177, Method: Composition-based stats.
Identities = 274/274 (100%), Positives = 274/274 (100%), Gaps = 0/274 (0%)
Query 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT
Sbjct 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
Query 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA
Sbjct 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
Query 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL
Sbjct 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
Query 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP
Sbjct 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
Query 241 PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL
Sbjct 241 PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
>sp|P0DTC3|AP3A_SARS2 ORF3a protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=3a PE=1 SV=1
Length=275
Score = 467 bits (1201), Expect = 2e-167, Method: Composition-based stats.
Identities = 200/275 (73%), Positives = 235/275 (85%), Gaps = 1/275 (0%)
Query 1 MDLFMRFFTLGSITAQPVKIDNASPASTVHATATIPLQASLPFGWLVIGVAFLAVFQSAT 60
MDLFMR FT+G++T + +I +A+P+ V ATATIP+QASLPFGWL++GVA LAVFQSA+
Sbjct 1 MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSAS 60
Query 61 KIIALNKRWQLALYKGFQFICNLLLLFVTIYSHLLLVAAGMEAQFLYLYALIYFLQCINA 120
KII L KRWQLAL KG F+CNLLLLFVT+YSHLLLVAAG+EA FLYLYAL+YFLQ IN
Sbjct 61 KIITLKKRWQLALSKGVHFVCNLLLLFVTVYSHLLLVAAGLEAPFLYLYALVYFLQSINF 120
Query 121 CRIIMRCWLCWKCKSKNPLLYDANYFVCWHTHNYDYCIPYNSVTDTIVVTEGDGISTPKL 180
RIIMR WLCWKC+SKNPLLYDANYF+CWHT+ YDYCIPYNSVT +IV+T GDG ++P
Sbjct 121 VRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPIS 180
Query 181 KEDYQIGGYSEDRHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDP 240
+ DYQIGGY+E SGVKD VV+H YFT YYQL STQ++TDTG+E+ TFFI+NK+V +P
Sbjct 181 EHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEP 240
Query 241 -PNVQIHTIDGSSGVANPAMDPIYDEPTTTTSVPL 274
+VQIHTIDGSSGV NP M+PIYDEPTTTTSVPL
Sbjct 241 EEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL 275
Lambda K H a alpha
0.319 0.143 0.462 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0438 0.140 1.90 42.6 43.6
Effective search space used: 22184730072
Search has CONVERGED!
Database: uniprot_sprot.fasta
Posted date: Apr 3, 2024 12:05 PM
Number of letters in database: 206,678,396
Number of sequences in database: 571,282
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40