PSIBLAST 2.11.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Stephen F.
Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005)
"Protein database searches using compositionally adjusted
substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: uniprot_sprot.fasta
571,282 sequences; 206,678,396 total letters
Results from round 1
Query= sp|P0DTD8|NS7B_SARS2 ORF7b protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=7b PE=1 SV=1
Length=43
Score E
Sequences producing significant alignments: (Bits) Value
sp|P0DTD8|NS7B_SARS2 ORF7b protein OS=Severe acute respiratory sy... 83.6 7e-23
sp|Q3I5I9|NS7B_BCRP3 Non-structural protein 7b OS=Bat coronavirus... 40.8 7e-06
sp|P0C5A9|NS7B_BC279 Non-structural protein 7b OS=Bat coronavirus... 40.8 7e-06
sp|Q7TFA1|NS7B_SARS Protein non-structural 7b OS=Severe acute res... 38.9 3e-05
sp|Q3LZX6|NS7B_BCHK3 Non-structural protein 7b OS=Bat coronavirus... 31.2 0.041
sp|Q9M883|SC5D2_ARATH Putative Delta(7)-sterol-C5(6)-desaturase 2... 30.0 0.33
sp|P79949|RFNG_XENLA Beta-1,3-N-acetylglucosaminyltransferase rad... 29.6 0.58
sp|P98164|LRP2_HUMAN Low-density lipoprotein receptor-related pro... 26.2 10.0
>sp|P0DTD8|NS7B_SARS2 ORF7b protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=7b PE=1 SV=1
Length=43
Score = 83.6 bits (205), Expect = 7e-23, Method: Compositional matrix adjust.
Identities = 43/43 (100%), Positives = 43/43 (100%), Gaps = 0/43 (0%)
Query 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA
Sbjct 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
>sp|Q3I5I9|NS7B_BCRP3 Non-structural protein 7b OS=Bat coronavirus
Rp3/2004 OX=349344 GN=7b PE=4 SV=1
Length=44
Score = 40.8 bits (94), Expect = 7e-06, Method: Compositional matrix adjust.
Identities = 36/43 (84%), Positives = 38/43 (88%), Gaps = 0/43 (0%)
Query 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
M EL+LIDFYLCFLAFLLFLVLIMLIIFWFSLELQD E C+
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDIEEPCNK 43
>sp|P0C5A9|NS7B_BC279 Non-structural protein 7b OS=Bat coronavirus
279/2005 OX=389167 GN=7b PE=4 SV=1
Length=44
Score = 40.8 bits (94), Expect = 7e-06, Method: Compositional matrix adjust.
Identities = 36/43 (84%), Positives = 38/43 (88%), Gaps = 0/43 (0%)
Query 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
M EL+LIDFYLCFLAFLLFLVLIMLIIFWFSLELQD E C+
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDIEEPCNK 43
>sp|Q7TFA1|NS7B_SARS Protein non-structural 7b OS=Severe acute
respiratory syndrome coronavirus OX=694009 GN=7b PE=1 SV=1
Length=44
Score = 38.9 bits (89), Expect = 3e-05, Method: Compositional matrix adjust.
Identities = 35/43 (81%), Positives = 37/43 (86%), Gaps = 0/43 (0%)
Query 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
M EL+LIDFYLCFLAFLLFLVLIMLIIFWFSLE+QD E C
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTK 43
>sp|Q3LZX6|NS7B_BCHK3 Non-structural protein 7b OS=Bat coronavirus
HKU3 OX=442736 GN=7b PE=4 SV=1
Length=44
Score = 31.2 bits (69), Expect = 0.041, Method: Compositional matrix adjust.
Identities = 34/43 (79%), Positives = 38/43 (88%), Gaps = 0/43 (0%)
Query 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
M EL+LIDFYLCFLAFLLFLVLIML+IFWFSLE+QD E C+
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLLIFWFSLEIQDIEEPCNK 43
>sp|Q9M883|SC5D2_ARATH Putative Delta(7)-sterol-C5(6)-desaturase
2 OS=Arabidopsis thaliana OX=3702 GN=HDF7 PE=3 SV=1
Length=279
Score = 30.0 bits (66), Expect = 0.33, Method: Compositional matrix adjust.
Identities = 13/29 (45%), Positives = 20/29 (69%), Gaps = 0/29 (0%)
Query 8 DFYLCFLAFLLFLVLIMLIIFWFSLELQD 36
+++LCFL L+LVL+ +I+W EL D
Sbjct 125 NWFLCFLYIALYLVLVEFMIYWVHKELHD 153
>sp|P79949|RFNG_XENLA Beta-1,3-N-acetylglucosaminyltransferase
radical fringe OS=Xenopus laevis OX=8355 GN=rfng PE=2 SV=1
Length=340
Score = 29.6 bits (65), Expect = 0.58, Method: Composition-based stats.
Identities = 16/37 (43%), Positives = 19/37 (51%), Gaps = 4/37 (11%)
Query 11 LCFLAFLLFLVLIMLIIFW----FSLELQDHNETCHA 43
+CFL FLL ++L I W S LQ N TC A
Sbjct 11 VCFLVFLLLCATVLLNISWRQRDSSQSLQHCNSTCSA 47
>sp|P98164|LRP2_HUMAN Low-density lipoprotein receptor-related
protein 2 OS=Homo sapiens OX=9606 GN=LRP2 PE=1 SV=3
Length=4655
Score = 26.2 bits (56), Expect = 10.0, Method: Composition-based stats.
Identities = 14/23 (61%), Positives = 17/23 (74%), Gaps = 1/23 (4%)
Query 19 FLVLIML-IIFWFSLELQDHNET 40
F+V+ ML I FSLEL DH+ET
Sbjct 2064 FIVVSMLSAIRGFSLELSDHSET 2086
Lambda K H a alpha
0.345 0.154 0.510 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 5121131652
Results from round 2
Query= sp|P0DTD8|NS7B_SARS2 ORF7b protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=7b PE=1 SV=1
Length=43
Score E
Sequences producing significant alignments: (Bits) Value
Sequences used in model and found again:
sp|P0DTD8|NS7B_SARS2 ORF7b protein OS=Severe acute respiratory sy... 44.0 3e-07
sp|Q3I5I9|NS7B_BCRP3 Non-structural protein 7b OS=Bat coronavirus... 33.2 0.006
sp|P0C5A9|NS7B_BC279 Non-structural protein 7b OS=Bat coronavirus... 33.2 0.006
sp|Q7TFA1|NS7B_SARS Protein non-structural 7b OS=Severe acute res... 32.5 0.013
Sequences not found previously or not previously below threshold:
sp|Q3LZX6|NS7B_BCHK3 Non-structural protein 7b OS=Bat coronavirus... 30.9 0.051
sp|Q9M883|SC5D2_ARATH Putative Delta(7)-sterol-C5(6)-desaturase 2... 26.3 8.0
>sp|P0DTD8|NS7B_SARS2 ORF7b protein OS=Severe acute respiratory
syndrome coronavirus 2 OX=2697049 GN=7b PE=1 SV=1
Length=43
Score = 44.0 bits (103), Expect = 3e-07, Method: Composition-based stats.
Identities = 43/43 (100%), Positives = 43/43 (100%), Gaps = 0/43 (0%)
Query 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA
Sbjct 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
>sp|Q3I5I9|NS7B_BCRP3 Non-structural protein 7b OS=Bat coronavirus
Rp3/2004 OX=349344 GN=7b PE=4 SV=1
Length=44
Score = 33.2 bits (75), Expect = 0.006, Method: Composition-based stats.
Identities = 36/43 (84%), Positives = 38/43 (88%), Gaps = 0/43 (0%)
Query 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
M EL+LIDFYLCFLAFLLFLVLIMLIIFWFSLELQD E C+
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDIEEPCNK 43
>sp|P0C5A9|NS7B_BC279 Non-structural protein 7b OS=Bat coronavirus
279/2005 OX=389167 GN=7b PE=4 SV=1
Length=44
Score = 33.2 bits (75), Expect = 0.006, Method: Composition-based stats.
Identities = 36/43 (84%), Positives = 38/43 (88%), Gaps = 0/43 (0%)
Query 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
M EL+LIDFYLCFLAFLLFLVLIMLIIFWFSLELQD E C+
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDIEEPCNK 43
>sp|Q7TFA1|NS7B_SARS Protein non-structural 7b OS=Severe acute
respiratory syndrome coronavirus OX=694009 GN=7b PE=1 SV=1
Length=44
Score = 32.5 bits (73), Expect = 0.013, Method: Composition-based stats.
Identities = 35/43 (81%), Positives = 37/43 (86%), Gaps = 0/43 (0%)
Query 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
M EL+LIDFYLCFLAFLLFLVLIMLIIFWFSLE+QD E C
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLIIFWFSLEIQDLEEPCTK 43
>sp|Q3LZX6|NS7B_BCHK3 Non-structural protein 7b OS=Bat coronavirus
HKU3 OX=442736 GN=7b PE=4 SV=1
Length=44
Score = 30.9 bits (69), Expect = 0.051, Method: Composition-based stats.
Identities = 34/43 (79%), Positives = 38/43 (88%), Gaps = 0/43 (0%)
Query 1 MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA 43
M EL+LIDFYLCFLAFLLFLVLIML+IFWFSLE+QD E C+
Sbjct 1 MNELTLIDFYLCFLAFLLFLVLIMLLIFWFSLEIQDIEEPCNK 43
>sp|Q9M883|SC5D2_ARATH Putative Delta(7)-sterol-C5(6)-desaturase
2 OS=Arabidopsis thaliana OX=3702 GN=HDF7 PE=3 SV=1
Length=279
Score = 26.3 bits (57), Expect = 8.0, Method: Composition-based stats.
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 0/35 (0%)
Query 4 LSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHN 38
L +++LCFL L+LVL+ +I+W EL D
Sbjct 121 LDHFNWFLCFLYIALYLVLVEFMIYWVHKELHDIK 155
Lambda K H a alpha
0.323 0.163 0.575 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0499 0.140 1.90 42.6 43.6
Effective search space used: 5121131652
Search has CONVERGED!
Database: uniprot_sprot.fasta
Posted date: Apr 3, 2024 12:05 PM
Number of letters in database: 206,678,396
Number of sequences in database: 571,282
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40