PSIBLAST 2.11.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Stephen F.
Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005)
"Protein database searches using compositionally adjusted
substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: uniprot_sprot.fasta
571,282 sequences; 206,678,396 total letters
Results from round 1
Query= sp|P0DTD3|ORF9C_SARS2 Putative ORF9c protein OS=Severe acute
respiratory syndrome coronavirus 2 OX=2697049 GN=9c PE=5 SV=1
Length=73
Score E
Sequences producing significant alignments: (Bits) Value
sp|P0DTD3|ORF9C_SARS2 Putative ORF9c protein OS=Severe acute resp... 145 2e-46
sp|Q3I5I5|Y14_BCRP3 Uncharacterized protein 14 OS=Bat coronavirus... 106 3e-31
sp|Q7TLC7|Y14_SARS Uncharacterized protein 14 OS=Severe acute res... 106 4e-31
sp|Q920P9|LY75_MESAU Lymphocyte antigen 75 OS=Mesocricetus auratu... 30.4 0.99
sp|O60449|LY75_HUMAN Lymphocyte antigen 75 OS=Homo sapiens OX=960... 30.0 1.4
sp|Q60767|LY75_MOUSE Lymphocyte antigen 75 OS=Mus musculus OX=100... 29.3 2.8
sp|Q0VCP1|KAD4_BOVIN Adenylate kinase 4, mitochondrial OS=Bos tau... 27.7 8.1
sp|H2KZZ6|TBC11_CAEEL Rab GTPase-activating protein tbc-11 OS=Cae... 27.7 8.3
sp|Q24167|SIMA_DROME Protein similar OS=Drosophila melanogaster O... 27.7 8.3
sp|B1W3B4|ARLY_STRGG Argininosuccinate lyase OS=Streptomyces gris... 27.7 8.6
sp|Q1B7P4|LYSX_MYCSS Lysylphosphatidylglycerol biosynthesis bifun... 27.7 9.5
sp|A1UHB3|LYSX_MYCSK Lysylphosphatidylglycerol biosynthesis bifun... 27.7 9.5
sp|P27144|KAD4_HUMAN Adenylate kinase 4, mitochondrial OS=Homo sa... 27.3 9.8
>sp|P0DTD3|ORF9C_SARS2 Putative ORF9c protein OS=Severe acute
respiratory syndrome coronavirus 2 OX=2697049 GN=9c PE=5 SV=1
Length=73
Score = 145 bits (365), Expect = 2e-46, Method: Compositional matrix adjust.
Identities = 73/73 (100%), Positives = 73/73 (100%), Gaps = 0/73 (0%)
Query 1 MLQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAM 60
MLQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAM
Sbjct 1 MLQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAM 60
Query 61 AVMLLLLCCCLTD 73
AVMLLLLCCCLTD
Sbjct 61 AVMLLLLCCCLTD 73
>sp|Q3I5I5|Y14_BCRP3 Uncharacterized protein 14 OS=Bat coronavirus
Rp3/2004 OX=349344 GN=ORF14 PE=4 SV=1
Length=70
Score = 106 bits (265), Expect = 3e-31, Method: Compositional matrix adjust.
Identities = 56/70 (80%), Positives = 58/70 (83%), Gaps = 0/70 (0%)
Query 1 MLQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAM 60
ML SCYNFLKEQHCQKASTQKGAEAAVKPLL HHVVA +QEIQL AAVGE+L LEWLA
Sbjct 1 MLPSCYNFLKEQHCQKASTQKGAEAAVKPLLALHHVVAVIQEIQLLAAVGEILQLEWLAE 60
Query 61 AVMLLLLCCC 70
AV L CC
Sbjct 61 AVKLPSRYCC 70
>sp|Q7TLC7|Y14_SARS Uncharacterized protein 14 OS=Severe acute
respiratory syndrome coronavirus OX=694009 GN=ORF14 PE=1 SV=1
Length=70
Score = 106 bits (264), Expect = 4e-31, Method: Compositional matrix adjust.
Identities = 54/70 (77%), Positives = 57/70 (81%), Gaps = 0/70 (0%)
Query 1 MLQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAM 60
ML CYNFLKEQHCQKASTQ+ AEAAVKPLL PHHVVA +QEIQL AAVGE+LLLEWLA
Sbjct 1 MLPPCYNFLKEQHCQKASTQREAEAAVKPLLAPHHVVAVIQEIQLLAAVGEILLLEWLAE 60
Query 61 AVMLLLLCCC 70
V L CC
Sbjct 61 VVKLPSRYCC 70
>sp|Q920P9|LY75_MESAU Lymphocyte antigen 75 OS=Mesocricetus auratus
OX=10036 GN=LY75 PE=2 SV=1
Length=1722
Score = 30.4 bits (67), Expect = 0.99, Method: Composition-based stats.
Identities = 17/61 (28%), Positives = 30/61 (49%), Gaps = 0/61 (0%)
Query 4 SCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVM 63
SCYNF+ ++ + TQK + + L +++ E + V +LL ++A VM
Sbjct 1254 SCYNFMITKNRHRTITQKEVHSLCQKLHSKAQILSIRNEEENNFVVEQLLYFNYIASWVM 1313
Query 64 L 64
L
Sbjct 1314 L 1314
>sp|O60449|LY75_HUMAN Lymphocyte antigen 75 OS=Homo sapiens OX=9606
GN=LY75 PE=1 SV=3
Length=1722
Score = 30.0 bits (66), Expect = 1.4, Method: Composition-based stats.
Identities = 16/60 (27%), Positives = 29/60 (48%), Gaps = 0/60 (0%)
Query 5 CYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVML 64
CYNF+ ++ A+TQ + L H+++ E + + +LL ++A VML
Sbjct 1255 CYNFIITKNRHMATTQDEVHTKCQKLNPKSHILSIRDEKENNFVLEQLLYFNYMASWVML 1314
>sp|Q60767|LY75_MOUSE Lymphocyte antigen 75 OS=Mus musculus OX=10090
GN=Ly75 PE=1 SV=2
Length=1723
Score = 29.3 bits (64), Expect = 2.8, Method: Composition-based stats.
Identities = 17/61 (28%), Positives = 29/61 (48%), Gaps = 0/61 (0%)
Query 4 SCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVM 63
SCYNF+ + K T + ++ + L H ++ E + V +LL ++A VM
Sbjct 1255 SCYNFMITNNRHKTVTPEEVQSTCEKLHSKAHSLSIRNEEENTFVVEQLLYFNYIASWVM 1314
Query 64 L 64
L
Sbjct 1315 L 1315
>sp|Q0VCP1|KAD4_BOVIN Adenylate kinase 4, mitochondrial OS=Bos
taurus OX=9913 GN=AK4 PE=2 SV=1
Length=223
Score = 27.7 bits (60), Expect = 8.1, Method: Composition-based stats.
Identities = 20/53 (38%), Positives = 30/53 (57%), Gaps = 5/53 (9%)
Query 7 NFLKEQHCQKASTQKGAEAAV---KPLLVPHHVVATVQEIQLQAAVGELLLLE 56
+FL+E KA+T+ G A K LLVP HV+ + ++L+ GE LL+
Sbjct 38 HFLREN--IKANTEVGDMAKQYIEKGLLVPDHVITRLMLLELENRRGEHWLLD 88
>sp|H2KZZ6|TBC11_CAEEL Rab GTPase-activating protein tbc-11 OS=Caenorhabditis
elegans OX=6239 GN=tbc-11 PE=1 SV=1
Length=934
Score = 27.7 bits (60), Expect = 8.3, Method: Composition-based stats.
Identities = 13/30 (43%), Positives = 18/30 (60%), Gaps = 0/30 (0%)
Query 20 QKGAEAAVKPLLVPHHVVATVQEIQLQAAV 49
QKGA+ L+VPH++ TV+ I Q V
Sbjct 43 QKGAQTINVTLVVPHNISGTVKLIDAQGKV 72
>sp|Q24167|SIMA_DROME Protein similar OS=Drosophila melanogaster
OX=7227 GN=sima PE=1 SV=2
Length=1507
Score = 27.7 bits (60), Expect = 8.3, Method: Composition-based stats.
Identities = 13/31 (42%), Positives = 16/31 (52%), Gaps = 0/31 (0%)
Query 10 KEQHCQKASTQKGAEAAVKPLLVPHHVVATV 40
KEQH Q A T+K E A P ++ TV
Sbjct 441 KEQHHQAAETEKEPEKAADPEIIAQETKETV 471
>sp|B1W3B4|ARLY_STRGG Argininosuccinate lyase OS=Streptomyces
griseus subsp. griseus (strain JCM 4626 / NBRC 13350) OX=455632
GN=argH PE=3 SV=1
Length=475
Score = 27.7 bits (60), Expect = 8.6, Method: Composition-based stats.
Identities = 16/46 (35%), Positives = 21/46 (46%), Gaps = 0/46 (0%)
Query 2 LQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQA 47
LQS L E H A + +P+L HHV+A VQ + A
Sbjct 142 LQSALVGLAEAHADVAMPGRTHLQHAQPVLFAHHVLAHVQSLSRDA 187
>sp|Q1B7P4|LYSX_MYCSS Lysylphosphatidylglycerol biosynthesis bifunctional
protein LysX OS=Mycobacterium sp. (strain MCS) OX=164756
GN=lysX PE=3 SV=1
Length=1112
Score = 27.7 bits (60), Expect = 9.5, Method: Composition-based stats.
Identities = 18/55 (33%), Positives = 23/55 (42%), Gaps = 8/55 (15%)
Query 6 YNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQE--------IQLQAAVGEL 52
Y L E QK + K +V PL PH +A V E ++L A EL
Sbjct 985 YEHLVEDRTQKPTFYKDFPTSVSPLTRPHRSIAGVAERWDLVAWGVELGTAYSEL 1039
>sp|A1UHB3|LYSX_MYCSK Lysylphosphatidylglycerol biosynthesis bifunctional
protein LysX OS=Mycobacterium sp. (strain KMS) OX=189918
GN=lysX PE=3 SV=1
Length=1112
Score = 27.7 bits (60), Expect = 9.5, Method: Composition-based stats.
Identities = 18/55 (33%), Positives = 23/55 (42%), Gaps = 8/55 (15%)
Query 6 YNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQE--------IQLQAAVGEL 52
Y L E QK + K +V PL PH +A V E ++L A EL
Sbjct 985 YEHLVEDRTQKPTFYKDFPTSVSPLTRPHRSIAGVAERWDLVAWGVELGTAYSEL 1039
>sp|P27144|KAD4_HUMAN Adenylate kinase 4, mitochondrial OS=Homo
sapiens OX=9606 GN=AK4 PE=1 SV=1
Length=223
Score = 27.3 bits (59), Expect = 9.8, Method: Composition-based stats.
Identities = 20/53 (38%), Positives = 29/53 (55%), Gaps = 5/53 (9%)
Query 7 NFLKEQHCQKASTQKGAEAAV---KPLLVPHHVVATVQEIQLQAAVGELLLLE 56
+FL+E KAST+ G A K LLVP HV+ + +L+ G+ LL+
Sbjct 38 HFLREN--IKASTEVGEMAKQYIEKSLLVPDHVITRLMMSELENRRGQHWLLD 88
Lambda K H a alpha
0.327 0.134 0.412 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 5067179768
Results from round 2
Query= sp|P0DTD3|ORF9C_SARS2 Putative ORF9c protein OS=Severe acute
respiratory syndrome coronavirus 2 OX=2697049 GN=9c PE=5 SV=1
Length=73
Score E
Sequences producing significant alignments: (Bits) Value
Sequences used in model and found again:
sp|Q7TLC7|Y14_SARS Uncharacterized protein 14 OS=Severe acute res... 106 4e-31
sp|Q3I5I5|Y14_BCRP3 Uncharacterized protein 14 OS=Bat coronavirus... 105 1e-30
sp|P0DTD3|ORF9C_SARS2 Putative ORF9c protein OS=Severe acute resp... 101 5e-29
Sequences not found previously or not previously below threshold:
sp|Q920P9|LY75_MESAU Lymphocyte antigen 75 OS=Mesocricetus auratu... 30.8 0.80
sp|O60449|LY75_HUMAN Lymphocyte antigen 75 OS=Homo sapiens OX=960... 30.4 1.0
sp|Q60767|LY75_MOUSE Lymphocyte antigen 75 OS=Mus musculus OX=100... 30.0 1.2
sp|P44240|VG47_HAEIN Mu-like prophage FluMu protein gp47 OS=Haemo... 28.1 5.8
>sp|Q7TLC7|Y14_SARS Uncharacterized protein 14 OS=Severe acute
respiratory syndrome coronavirus OX=694009 GN=ORF14 PE=1 SV=1
Length=70
Score = 106 bits (264), Expect = 4e-31, Method: Composition-based stats.
Identities = 54/70 (77%), Positives = 57/70 (81%), Gaps = 0/70 (0%)
Query 1 MLQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAM 60
ML CYNFLKEQHCQKASTQ+ AEAAVKPLL PHHVVA +QEIQL AAVGE+LLLEWLA
Sbjct 1 MLPPCYNFLKEQHCQKASTQREAEAAVKPLLAPHHVVAVIQEIQLLAAVGEILLLEWLAE 60
Query 61 AVMLLLLCCC 70
V L CC
Sbjct 61 VVKLPSRYCC 70
>sp|Q3I5I5|Y14_BCRP3 Uncharacterized protein 14 OS=Bat coronavirus
Rp3/2004 OX=349344 GN=ORF14 PE=4 SV=1
Length=70
Score = 105 bits (261), Expect = 1e-30, Method: Composition-based stats.
Identities = 56/70 (80%), Positives = 58/70 (83%), Gaps = 0/70 (0%)
Query 1 MLQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAM 60
ML SCYNFLKEQHCQKASTQKGAEAAVKPLL HHVVA +QEIQL AAVGE+L LEWLA
Sbjct 1 MLPSCYNFLKEQHCQKASTQKGAEAAVKPLLALHHVVAVIQEIQLLAAVGEILQLEWLAE 60
Query 61 AVMLLLLCCC 70
AV L CC
Sbjct 61 AVKLPSRYCC 70
>sp|P0DTD3|ORF9C_SARS2 Putative ORF9c protein OS=Severe acute
respiratory syndrome coronavirus 2 OX=2697049 GN=9c PE=5 SV=1
Length=73
Score = 101 bits (251), Expect = 5e-29, Method: Composition-based stats.
Identities = 73/73 (100%), Positives = 73/73 (100%), Gaps = 0/73 (0%)
Query 1 MLQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAM 60
MLQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAM
Sbjct 1 MLQSCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAM 60
Query 61 AVMLLLLCCCLTD 73
AVMLLLLCCCLTD
Sbjct 61 AVMLLLLCCCLTD 73
>sp|Q920P9|LY75_MESAU Lymphocyte antigen 75 OS=Mesocricetus auratus
OX=10036 GN=LY75 PE=2 SV=1
Length=1722
Score = 30.8 bits (68), Expect = 0.80, Method: Composition-based stats.
Identities = 17/65 (26%), Positives = 31/65 (48%), Gaps = 0/65 (0%)
Query 4 SCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVM 63
SCYNF+ ++ + TQK + + L +++ E + V +LL ++A VM
Sbjct 1254 SCYNFMITKNRHRTITQKEVHSLCQKLHSKAQILSIRNEEENNFVVEQLLYFNYIASWVM 1313
Query 64 LLLLC 68
L +
Sbjct 1314 LGVTY 1318
>sp|O60449|LY75_HUMAN Lymphocyte antigen 75 OS=Homo sapiens OX=9606
GN=LY75 PE=1 SV=3
Length=1722
Score = 30.4 bits (67), Expect = 1.0, Method: Composition-based stats.
Identities = 16/64 (25%), Positives = 30/64 (47%), Gaps = 0/64 (0%)
Query 5 CYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVML 64
CYNF+ ++ A+TQ + L H+++ E + + +LL ++A VML
Sbjct 1255 CYNFIITKNRHMATTQDEVHTKCQKLNPKSHILSIRDEKENNFVLEQLLYFNYMASWVML 1314
Query 65 LLLC 68
+
Sbjct 1315 GITY 1318
>sp|Q60767|LY75_MOUSE Lymphocyte antigen 75 OS=Mus musculus OX=10090
GN=Ly75 PE=1 SV=2
Length=1723
Score = 30.0 bits (66), Expect = 1.2, Method: Composition-based stats.
Identities = 17/65 (26%), Positives = 30/65 (46%), Gaps = 0/65 (0%)
Query 4 SCYNFLKEQHCQKASTQKGAEAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVM 63
SCYNF+ + K T + ++ + L H ++ E + V +LL ++A VM
Sbjct 1255 SCYNFMITNNRHKTVTPEEVQSTCEKLHSKAHSLSIRNEEENTFVVEQLLYFNYIASWVM 1314
Query 64 LLLLC 68
L +
Sbjct 1315 LGITY 1319
>sp|P44240|VG47_HAEIN Mu-like prophage FluMu protein gp47 OS=Haemophilus
influenzae (strain ATCC 51907 / DSM 11121 / KW20
/ Rd) OX=71421 GN=HI_1520 PE=3 SV=1
Length=355
Score = 28.1 bits (61), Expect = 5.8, Method: Composition-based stats.
Identities = 11/34 (32%), Positives = 18/34 (53%), Gaps = 0/34 (0%)
Query 29 PLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAV 62
P L H+V+ + I + GE + L+WLA +
Sbjct 24 PTLKRHNVIGVINRICAALSAGEHMHLDWLARQI 57
Lambda K H a alpha
0.314 0.134 0.441 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0412 0.140 1.90 42.6 43.6
Effective search space used: 5067179768
Search has CONVERGED!
Database: uniprot_sprot.fasta
Posted date: Apr 3, 2024 12:05 PM
Number of letters in database: 206,678,396
Number of sequences in database: 571,282
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40