[Multiple Alignment(many alignments)]
[Alignment Bar(many alignments)]
[show plain BLAST file]
BLASTP 2.11.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: unitmol_20240403.fasta
829,018 sequences; 237,935,689 total letters
Query= sp|P59596|VME1_SARS Membrane protein OS=Severe acute respiratory
syndrome coronavirus OX=694009 GN=M PE=1 SV=1
Length=221
Score E
Sequences producing significant alignments: (Bits) Value
7vgr_E A Membrane protein 377 2e-133
7vgr_F B Membrane protein 377 2e-133
7vgs_D B Membrane protein 374 8e-132
7vgs_A A Membrane protein 374 8e-132
8ctk_A A Membrane protein 359 2e-126
8ctk_B B Membrane protein 359 2e-126
7y9b_A A Membrane protein 172 8e-53
7y9b_C C Membrane protein 172 8e-53
7y9b_B B Membrane protein 172 8e-53
7y9b_D D Membrane protein 172 8e-53
7y96_B B Green fluorescent protein,Membrane protein 84.0 8e-18
7y96_A A Green fluorescent protein,Membrane protein 80.1 2e-16
8cme_F F Membrane protein 30.0 1.4
8cme_I I Membrane protein 30.0 1.4
8cme_C C Membrane protein 30.0 1.4
>7vgr_E A Membrane protein
Length=246
Score = 377 bits (969), Expect = 2e-133, Method: Compositional matrix adjust.
Identities = 181/198 (91%), Positives = 192/198 (97%), Gaps = 0/198 (0%)
Query 8 TVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLA 67
TVEELK+LLEQWNLVIGFLFL WI LLQFAY+NRNRFLYIIKL+FLWLLWPVTLACFVLA
Sbjct 33 TVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLA 92
Query 68 AVYRINWVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTI 127
AVYRINW+TGGIAIAMAC+VGLMWLSYF+ASFRLFARTRSMWSFNPETNILLNVPL GTI
Sbjct 93 AVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTI 152
Query 128 VTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVG 187
+TRPL+ESELVIGAVI+RGHLR+AGH LGRCDIKDLPKEITVATSRTLSYYKLGASQRV
Sbjct 153 LTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVA 212
Query 188 TDSGFAAYNRYRIGNYKL 205
DSGFAAY+RYRIGNYKL
Sbjct 213 GDSGFAAYSRYRIGNYKL 230
>7vgr_F B Membrane protein
Length=246
Score = 377 bits (969), Expect = 2e-133, Method: Compositional matrix adjust.
Identities = 181/198 (91%), Positives = 192/198 (97%), Gaps = 0/198 (0%)
Query 8 TVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLA 67
TVEELK+LLEQWNLVIGFLFL WI LLQFAY+NRNRFLYIIKL+FLWLLWPVTLACFVLA
Sbjct 33 TVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLA 92
Query 68 AVYRINWVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTI 127
AVYRINW+TGGIAIAMAC+VGLMWLSYF+ASFRLFARTRSMWSFNPETNILLNVPL GTI
Sbjct 93 AVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTI 152
Query 128 VTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVG 187
+TRPL+ESELVIGAVI+RGHLR+AGH LGRCDIKDLPKEITVATSRTLSYYKLGASQRV
Sbjct 153 LTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVA 212
Query 188 TDSGFAAYNRYRIGNYKL 205
DSGFAAY+RYRIGNYKL
Sbjct 213 GDSGFAAYSRYRIGNYKL 230
>7vgs_D B Membrane protein
Length=246
Score = 374 bits (959), Expect = 8e-132, Method: Compositional matrix adjust.
Identities = 179/196 (91%), Positives = 190/196 (97%), Gaps = 0/196 (0%)
Query 8 TVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLA 67
TVEELK+LLEQWNLVIGFLFL WI LLQFAY+NRNRFLYIIKL+FLWLLWPVTLACFVLA
Sbjct 33 TVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLA 92
Query 68 AVYRINWVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTI 127
AVYRINW+TGGIAIAMAC+VGLMWLSYF+ASFRLFARTRSMWSFNPETNILLNVPL GTI
Sbjct 93 AVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTI 152
Query 128 VTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVG 187
+TRPL+ESELVIGAVI+RGHLR+AGH LGRCDIKDLPKEITVATSRTLSYYKLGASQRV
Sbjct 153 LTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVA 212
Query 188 TDSGFAAYNRYRIGNY 203
DSGFAAY+RYRIGNY
Sbjct 213 GDSGFAAYSRYRIGNY 228
>7vgs_A A Membrane protein
Length=246
Score = 374 bits (959), Expect = 8e-132, Method: Compositional matrix adjust.
Identities = 179/196 (91%), Positives = 190/196 (97%), Gaps = 0/196 (0%)
Query 8 TVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLA 67
TVEELK+LLEQWNLVIGFLFL WI LLQFAY+NRNRFLYIIKL+FLWLLWPVTLACFVLA
Sbjct 33 TVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLA 92
Query 68 AVYRINWVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTI 127
AVYRINW+TGGIAIAMAC+VGLMWLSYF+ASFRLFARTRSMWSFNPETNILLNVPL GTI
Sbjct 93 AVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTI 152
Query 128 VTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVG 187
+TRPL+ESELVIGAVI+RGHLR+AGH LGRCDIKDLPKEITVATSRTLSYYKLGASQRV
Sbjct 153 LTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVA 212
Query 188 TDSGFAAYNRYRIGNY 203
DSGFAAY+RYRIGNY
Sbjct 213 GDSGFAAYSRYRIGNY 228
>8ctk_A A Membrane protein
Length=231
Score = 359 bits (922), Expect = 2e-126, Method: Compositional matrix adjust.
Identities = 172/188 (91%), Positives = 182/188 (97%), Gaps = 0/188 (0%)
Query 16 LEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWV 75
LEQWNLVIGFLFL WI LLQFAY+NRNRFLYIIKL+FLWLLWPVTLACFVLAAVYRINW+
Sbjct 17 LEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLAAVYRINWI 76
Query 76 TGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMES 135
TGGIAIAMAC+VGLMWLSYF+ASFRLFARTRSMWSFNPETNILLNVPL GTI+TRPL+ES
Sbjct 77 TGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTILTRPLLES 136
Query 136 ELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVGTDSGFAAY 195
ELVIGAVI+RGHLR+AGH LGRCDIKDLPKEITVATSRTLSYYKLGASQRV DSGFAAY
Sbjct 137 ELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAY 196
Query 196 NRYRIGNY 203
+RYRIGNY
Sbjct 197 SRYRIGNY 204
>8ctk_B B Membrane protein
Length=231
Score = 359 bits (922), Expect = 2e-126, Method: Compositional matrix adjust.
Identities = 172/188 (91%), Positives = 182/188 (97%), Gaps = 0/188 (0%)
Query 16 LEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWV 75
LEQWNLVIGFLFL WI LLQFAY+NRNRFLYIIKL+FLWLLWPVTLACFVLAAVYRINW+
Sbjct 17 LEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLAAVYRINWI 76
Query 76 TGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMES 135
TGGIAIAMAC+VGLMWLSYF+ASFRLFARTRSMWSFNPETNILLNVPL GTI+TRPL+ES
Sbjct 77 TGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTILTRPLLES 136
Query 136 ELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVGTDSGFAAY 195
ELVIGAVI+RGHLR+AGH LGRCDIKDLPKEITVATSRTLSYYKLGASQRV DSGFAAY
Sbjct 137 ELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAY 196
Query 196 NRYRIGNY 203
+RYRIGNY
Sbjct 197 SRYRIGNY 204
>7y9b_A A Membrane protein
Length=220
Score = 172 bits (436), Expect = 8e-53, Method: Compositional matrix adjust.
Identities = 85/185 (46%), Positives = 117/185 (63%), Gaps = 0/185 (0%)
Query 14 QLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRIN 73
+L++ WN +FL ++LQ+ Y +R+ F+Y+IK+ LWLLWP ++A + AVY I+
Sbjct 15 RLVKDWNFTWSVVFLLITIVLQYGYPSRSMFVYVIKMFVLWLLWPASMALSIFCAVYPID 74
Query 74 WVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLM 133
+ I+ +A MW+SYFV S RLF RT S WSFNPE+N LLNVP+ GT V RPL+
Sbjct 75 LASQIISGILAATSCAMWISYFVQSIRLFMRTGSWWSFNPESNCLLNVPIGGTTVVRPLV 134
Query 134 ESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVGTDSGFA 193
E + AV+ G+L+MAG G CD + LP E+TVA L K+ Q GT+SG A
Sbjct 135 EDSTSVTAVVTDGYLKMAGMHFGACDFQRLPSEVTVAKPNVLIALKMIKRQAYGTNSGVA 194
Query 194 AYNRY 198
Y+RY
Sbjct 195 IYHRY 199
>7y9b_C C Membrane protein
Length=220
Score = 172 bits (436), Expect = 8e-53, Method: Compositional matrix adjust.
Identities = 85/185 (46%), Positives = 117/185 (63%), Gaps = 0/185 (0%)
Query 14 QLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRIN 73
+L++ WN +FL ++LQ+ Y +R+ F+Y+IK+ LWLLWP ++A + AVY I+
Sbjct 15 RLVKDWNFTWSVVFLLITIVLQYGYPSRSMFVYVIKMFVLWLLWPASMALSIFCAVYPID 74
Query 74 WVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLM 133
+ I+ +A MW+SYFV S RLF RT S WSFNPE+N LLNVP+ GT V RPL+
Sbjct 75 LASQIISGILAATSCAMWISYFVQSIRLFMRTGSWWSFNPESNCLLNVPIGGTTVVRPLV 134
Query 134 ESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVGTDSGFA 193
E + AV+ G+L+MAG G CD + LP E+TVA L K+ Q GT+SG A
Sbjct 135 EDSTSVTAVVTDGYLKMAGMHFGACDFQRLPSEVTVAKPNVLIALKMIKRQAYGTNSGVA 194
Query 194 AYNRY 198
Y+RY
Sbjct 195 IYHRY 199
>7y9b_B B Membrane protein
Length=220
Score = 172 bits (436), Expect = 8e-53, Method: Compositional matrix adjust.
Identities = 85/185 (46%), Positives = 117/185 (63%), Gaps = 0/185 (0%)
Query 14 QLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRIN 73
+L++ WN +FL ++LQ+ Y +R+ F+Y+IK+ LWLLWP ++A + AVY I+
Sbjct 15 RLVKDWNFTWSVVFLLITIVLQYGYPSRSMFVYVIKMFVLWLLWPASMALSIFCAVYPID 74
Query 74 WVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLM 133
+ I+ +A MW+SYFV S RLF RT S WSFNPE+N LLNVP+ GT V RPL+
Sbjct 75 LASQIISGILAATSCAMWISYFVQSIRLFMRTGSWWSFNPESNCLLNVPIGGTTVVRPLV 134
Query 134 ESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVGTDSGFA 193
E + AV+ G+L+MAG G CD + LP E+TVA L K+ Q GT+SG A
Sbjct 135 EDSTSVTAVVTDGYLKMAGMHFGACDFQRLPSEVTVAKPNVLIALKMIKRQAYGTNSGVA 194
Query 194 AYNRY 198
Y+RY
Sbjct 195 IYHRY 199
>7y9b_D D Membrane protein
Length=220
Score = 172 bits (436), Expect = 8e-53, Method: Compositional matrix adjust.
Identities = 85/185 (46%), Positives = 117/185 (63%), Gaps = 0/185 (0%)
Query 14 QLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRIN 73
+L++ WN +FL ++LQ+ Y +R+ F+Y+IK+ LWLLWP ++A + AVY I+
Sbjct 15 RLVKDWNFTWSVVFLLITIVLQYGYPSRSMFVYVIKMFVLWLLWPASMALSIFCAVYPID 74
Query 74 WVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLM 133
+ I+ +A MW+SYFV S RLF RT S WSFNPE+N LLNVP+ GT V RPL+
Sbjct 75 LASQIISGILAATSCAMWISYFVQSIRLFMRTGSWWSFNPESNCLLNVPIGGTTVVRPLV 134
Query 134 ESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVGTDSGFA 193
E + AV+ G+L+MAG G CD + LP E+TVA L K+ Q GT+SG A
Sbjct 135 EDSTSVTAVVTDGYLKMAGMHFGACDFQRLPSEVTVAKPNVLIALKMIKRQAYGTNSGVA 194
Query 194 AYNRY 198
Y+RY
Sbjct 195 IYHRY 199
>7y96_B B Green fluorescent protein,Membrane protein
Length=344
Score = 84.0 bits (206), Expect = 8e-18, Method: Compositional matrix adjust.
Identities = 41/86 (48%), Positives = 54/86 (63%), Gaps = 0/86 (0%)
Query 114 ETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSR 173
E+N LLNVP+ GT V RPL+E + AV+ G+L+MAG G CD + LP E+TVA
Sbjct 147 ESNCLLNVPIGGTTVVRPLVEDSTSVTAVVTDGYLKMAGMHFGACDFQRLPSEVTVAKPN 206
Query 174 TLSYYKLGASQRVGTDSGFAAYNRYR 199
L K+ Q GT+SG A Y+RY+
Sbjct 207 VLIALKMIKRQAYGTNSGVAIYHRYK 232
>7y96_A A Green fluorescent protein,Membrane protein
Length=344
Score = 80.1 bits (196), Expect = 2e-16, Method: Compositional matrix adjust.
Identities = 40/84 (48%), Positives = 52/84 (62%), Gaps = 0/84 (0%)
Query 114 ETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSR 173
E+N LLNVP+ GT V RPL+E + AV+ G+L+MAG G CD + LP E+TVA
Sbjct 147 ESNCLLNVPIGGTTVVRPLVEDSTSVTAVVTDGYLKMAGMHFGACDFQRLPSEVTVAKPN 206
Query 174 TLSYYKLGASQRVGTDSGFAAYNR 197
L K+ Q GT+SG A Y+R
Sbjct 207 VLIALKMIKRQAYGTNSGVAIYHR 230
>8cme_F F Membrane protein
Length=15
Score = 30.0 bits (66), Expect = 1.4, Method: Composition-based stats.
Identities = 13/15 (87%), Positives = 13/15 (87%), Gaps = 0/15 (0%)
Query 175 LSYYKLGASQRVGTD 189
LSYYKLGASQRV D
Sbjct 1 LSYYKLGASQRVAGD 15
>8cme_I I Membrane protein
Length=15
Score = 30.0 bits (66), Expect = 1.4, Method: Composition-based stats.
Identities = 13/15 (87%), Positives = 13/15 (87%), Gaps = 0/15 (0%)
Query 175 LSYYKLGASQRVGTD 189
LSYYKLGASQRV D
Sbjct 1 LSYYKLGASQRVAGD 15
>8cme_C C Membrane protein
Length=15
Score = 30.0 bits (66), Expect = 1.4, Method: Composition-based stats.
Identities = 13/15 (87%), Positives = 13/15 (87%), Gaps = 0/15 (0%)
Query 175 LSYYKLGASQRVGTD 189
LSYYKLGASQRV D
Sbjct 1 LSYYKLGASQRVAGD 15
Lambda K H a alpha
0.328 0.140 0.440 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 15346757159
Database: unitmol_20240403.fasta
Posted date: Apr 4, 2024 8:53 PM
Number of letters in database: 237,935,689
Number of sequences in database: 829,018
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40